Steven Thorat

Innovative biotechnology specialist with a strong academic and Bioinformatics background and hands-on experience in plant and microbial research. Skilled Senior Lab Technician and Sex Sorting Specialist with a successful history in cattle genetics, overseeing lab operations, leading teams, and contributing to scientific advancements. Combines deep technical knowledge in biotech research with proven leadership, collaboration, and communication abilities. Passionate about applying scientific expertise to drive impactful solutions in the biotech industry.

- Python for Genomic Data Science from JOHNS HOPKINS University [1 March- 12 April 2022].

- Next-Generation sequencing for SARS-COV-2 from future learn Bill and Melinda Gates foundation [January2020].

- Manav scientific reading and comprehension self-assessment module [sept- oct 2021].

- Mr. Biotech 2020, Ahmednagar College, for overall performance, academic improvement, team coordination, sincerity, extracurricular activities, and good etiquettes. [20th Feb 2020]

- English Proficiency test: IELTS exam overall 6.5 band [Feb 2024]

• PG Diploma course in Industrial Biotechnology [December 2021 to May2022]

- Covered 7 modules as follows:

1 - Genetic Engineering: RFLP, PCR, Molecular biology concepts.

2 - Plant Tissue Culture: Totipotency, Lab Organization, Aseptic Inoculation Techniques, and Applications of Plant Tissue Culture.

3 - Fermentation Technology: Got a Professional experience into Alcohol production, Sterilization Methods, Instrumentation, Process Control, Downstream Processing.

4 - Bio-fertilizers and Bio-pesticides Production: Media Preparation and Sterilization Techniques, Scale up, Formulation.

5 - Bioinformatics: Got a Professional experience into Protein and Nucleic Acid Sequence/Structural databases, Sequence Alignment, Phylogenetic Tree Construction, Protein Structure Prediction, Molecular Docking, Applications [Drug designing, Plant modeling, Genomics, etc.]

6 - Microbial QC and Testing: Quality Assurance and Quality Assurance Validation, Special microbial Tests [Polymerase Chain Reaction].

7 - Scientific Writing and Medical Writing: Research/Review paper writing, Introduction to Various Methods [hypothesis, Gather the data, analyses, Draw conclusions].

• Python for Genomic Data Science from JOHNS HOPKINS University [1 March- 12 April 2022].
• Next-Generation sequencing for SARS-COV-2 from future learn Bill and Melinda Gates foundation [January2020].
• Manav scientific reading and comprehension self-assessment module [sept- oct 2021].
• Mr. Biotech 2020, Ahmednagar College, for overall performance, academic improvement, team coordination, sincerity, extracurricular activities, and good etiquettes. [20th Feb 2020]
• English Proficiency test: IELTS exam overall 6.5 band [Feb 2024]

- Pratik, Naikwadi, Co-founder, p.naikwadi22@abdn.ac.uk, pratik@zygotelink.com, +44(786)704-3390, Zygotelink.ltd

- Mr. Yogesh Arjun Asst. Lab manager at ST (Sexing Technologies) Genetics, India.  Email: arjunyogesh2761999@gmail.com   Phone: +91 7219734292  

- Dr. Vishal Dawkar Research Scientist at MITCON Institute, Pune, India.   Email: vishal.dawkar@mitconindia.com   Phone: +91 8805183596, +91 2066289451   

- Dr. Matthew Uzagare Senior General Manager in R and D at Arch Pharm labs Ltd.   Email: Matthew.uzagare@archpharmalabs.com

• Title: Information on milk and dairy Industry. [Mauli Milk and Dairy Industry Rahuri, India, December 2018]
• Title: Fermentation technology. [Sula Wine Yard, Nashik, India, Feb-2020]
• Title: Different types of bio fertilizers and pesticides used in Agriculture. [Ashwamedh Biotechnology Industry, Kopargaon, India, Nov-2019]

• 1. ELISA, Spectrophotometer, Basic fermentation techniques like, downstream processing isolation of secondary metabolites from plant leaf fluorescent microscopy, sub culturing, staining techniques- Gram staining, and capsule staining, DNSA method for sugar estimation.
• 2. Callus culture and suspension culture technique of various plant species, Initiation of shoot tip and axillary bud culture and subculture, effect of plant growth regulators on in-vitro response. [Supervision: Ms. Rajashri Bhope and Ms. Komal Ma’am]
• 3. “Phytochemical analysis and Antimicrobial potential of Mentha spicata L.” [Supervision: Dr. Vishal Dawkar]
• worked on the medical importance of Mentha spicata L.
• Worked on phytochemical analysis to detect Secondary metabolites in mint extract.
• Isolated secondary metabolites
• Got good hands on SDS page for separation of proteins.
• Performed antifungal, sugar-protein estimation test and antimicrobial tests.
• Wet lab techniques: Chromatography, Microscopy, Spectrophotometry, Centrifugation, Buffer Solution Preparation, RNA, DNA Isolation, Rt-PCR, PCR, 2D - Gel Electrophoresis, SDS PAGE, Flow cytometry. [Supervision: Dr. Shiny ma’am]
• Dry lab techniques: Phylogenetic Tree Construction, Protein Structure Prediction, Molecular Docking, Applications like Drug designing, Plant modelling, etc. [Supervision: Dr. Norine ma’am]

Cricket - Team player, Captain, discipline, strategy and resilience

Group Singing - Patient, nurturing, and detail-oriented in cultivation growth.

Talking with strangers- Strong communicator adept at building rapport. 

Movies - Storytelling enthusiast with sharp observational skills.

Social work - Empathetic leader committed to community impact

Overall a well-bounded professional blending passion, teamwork, people skills and practical knowledge person.