Subhashini Ravi
Hyderabad
Summary
Biomedical researcher from IIT Hyderabad, specializing in 3D bioprinting, biomaterials, and drug delivery. Extensive experience in EV isolation, purification, and characterization, particularly from MSCs, with a strong interest in their therapeutic applications. Skilled in engineering EV-integrated scaffolds for controlled and targeted release in regenerative contexts, including bone tissue engineering. Proficient in stem cell culture, scaffold development, and preclinical in vitro/in vivo models.
Overview
12
12
years of professional experience
Work History
Senior Research Associate
UR Advanced Therapeutics
Hyderabad
06.2024 - Current
- Isolation and Expansion of Primary Cells: Expertise in isolating umbilical cord-derived mesenchymal stromal cells (UMSCs) from Wharton's jelly and optimizing culture conditions for expansion
- Microcarrier-based Cell Culture: Scalable UMSC expansion using Cytodex 3 microcarriers in Bioflow 320 bioreactor systems
- Exosome Research: Isolation, purification, and characterization of extracellular vesicles (EVs) from UMSCs using PEG method, NTA, TEM, Western blot, and ELISA
- 3D Bioprinting & Biomaterials Engineering: Development of 3D bioprintable hydrogels like PEGDA, GelMA, COLMA and EV-functionalized biomaterials
- In Vitro Assays: Spheroid culture, cytotoxicity testing, anti-cancer drug screening etc.
- Molecular & Cellular Techniques: Flow cytometry, RT-qPCR, Western blotting, immunofluorescence (IF), immunohistochemistry (IHC), and histological analysis
- Animal Handling & Preclinical Evaluation: Experience with rodent models (mouse and rat) for scaffold implantation, exosome administration, and toxicological studies (acute and subacute)
- Microscopy & Imaging: Fluorescence and confocal microscopy for scaffold-cell interaction and tissue-level analysis
- Data Analysis & Scientific Communication: Statistical analysis, report writing, manuscript preparation, and grant proposal drafting
PhD Research Scholar
Indian Institute of Technology, Hyderabad
Hyderabad
07.2018 - 10.2025
- Stem cell culture, Cartilage Tissue Engineering, Regenerative Medicine
- Biomaterials, Photo cross-linkable hydrogels, Drug Delivery, 3D Bioprinting
- Spheroid culture, Anti-cancer drug screening, In vitro drug testing.
- Thesis Submitted
Assistant Professor
DCRM Pharmacy College
01.2014 - 06.2018
- Lectures to B. Pharmacy students- Human Anatomy & Physiology, Pharmacology
- Laboratory sessions for graduate and postgraduate students
Education
Ph.D. - Biomedical Engineering
Indian Institute of Technology, Hyderabad
Hyderabad07.2025
M. Pharmacy - Pharmacology, Pre-clinical Animal Testing
Bapatla College of Pharmacy
Bapatla12.2013
B. Pharmacy - Pharmacology, Pharmaceutical Technology, Pharm. Analysis
Vishwa Bharathi College of Pharmaceutical Sciences
Guntur05.2011
Skills
Primary stem cell isolation and mammalian cell culture techniques (2D, 3D spheroid, and scaffold-based cultures)
Extracellular vesicle (EV) isolation and characterization: ultracentrifugation, NTA, TEM, Western blot
Flow cytometry, fluorescence microscopy, and confocal imaging techniques
Immunofluorescence (IF) and immunohistochemistry (IHC) for tissue and cellular analysis
Histological processing and staining, including H&E and specialized stains for tissue evaluation
Molecular biology techniques: RT-qPCR, Western blotting, and ELISA
In vitro drug screening and cytotoxicity assays using cancer and stem cell-derived models
Preclinical animal handling (mouse and rat): scaffold implantation, EV administration, sample collection
Toxicological evaluations: acute and subacute toxicity studies in rodent models
Experience with biomaterials and nanotechnology for drug/EV delivery and regenerative applications
Scaffold fabrication: hydrogel-based systems, microspheres, and 3D bioprinted constructs
Design and execution of in vitro/in vivo models for bone regeneration and tissue repair studies
Scientific writing, data analysisusing Graph pad prism and origin, and presentation of research findings in peer-reviewed formats
Awards
Qualified in CSIR NET JRF in June 2017 and got AIR 96
Qualified in TS SET in the year 2017
Qualified in AP SET in the year 2016
Qualified in GPAT in the year 2011 and got AIR 5642
Travel grant from IIT Hyderabad to attend the TERMIS EU 2022, 28.06-01.07.2022, Krakow, Poland,
Received an encouragement award for the presentation titled '3D Bioprintable Hypoxia Mimicking PEG Based Nano-Bioink for Cartilage Tissue Engineering' at the 2022 TERMIS-AP Virtual Student Paper Contest, December 17-18, 2022 (virtual presentation)
Publications
- Subhashini Ravi, Chokkakula L.P. Pavithra, Pravin Shankar Giri, Gayathri Korra, Suhash Ranjan Dey, Subha Narayan Rath “3D Bioprintable Hypoxia Mimicking PEG Based Nano-Bioink for Cartilage Tissue Engineering”. ACS Appl. Mater. Interfaces 15 (2023):19921–19936. (Impact factor:10.3).
- Subhashini, Ravi, and D. Ramesh Reddy. "Formulation and evaluation of Domperidone fast dissolving tablets using Plantago ovata mucilage." International Journal of Pharmaceutical Sciences and Research 4.9 (2013): 3489. (Impact factor:3.2)
- Subhashini Ravi, Gaddamedi Sreevani, Harsha Gopal Agrawal, Ambikesh soni, Ashutosh Kumar Mishra, Subha Narayan Rath “Design, Synthesis, Biological Evaluation and Molecular Docking Studies of N3-Functionalized TZD-Indole Conjugates as Potential Anticancer Agents. ChemistrySelect 17 (2024):1-8 (Impact factor:2.3)
- Subhashini Ravi, Chokkakula L.P. Pavithra, Suhash Ranjan Dey, Subha Narayan Rath “Fabrication of Hypoxia Mimicking Supramolecular Hydrogels for Cartilage Repair.” ACS Applied Biomaterials. (Impact factor:4.7).
- Sankar, Sharanya, Viraj Mehta, Subhashini Ravi, Chandra Shekhar Sharma, and Subha Narayan Rath. "A novel design of a microfluidic platform for metronomic combinatorial chemotherapy drug screening based on 3D tumor spheroid model." Biomedical Microdevices 23 (2021):1-10. (Impact factor:3.7).
- Subhashini Ravi, Chokkakula L.P. Pavithra, Pravin Shankar Giri, Gayathri Korra, Suhash Ranjan Dey
- Subha Narayan Rath
- Novel Hypoxia Mimicking PEG-Based Nano-Bioink for Cartilage Regeneration Application, TERMIS EU 2022, 28.06-01.07.2022, Krakow, Poland. (Oral presentation)
- “3D Bioprintable Hypoxia Mimicking PEG Based Nano-Bioink for Cartilage Tissue Engineering” 2022 TERMIS-AP Virtual Student Paper Contest,17.12-18.12.2022 (Virtual presentation)
- “3D Bioprintable Hypoxia Mimicking PEG Based Nano-Bioink for Cartilage Tissue Engineering”, HYSCI, 2023,17.03.2023, NIAB, Hyderabad. (Poster presentation and Flash talk)
- “Co Nanowires Embedded Hypoxia Mimicking PEG Based Bioink for Cartilage Tissue Engineering”, 2nd World Conference on Material Science and Nanotechnology. Brussels, Belgium 26-28 May 2023. (Virtual presentation)
Timeline
Senior Research Associate
UR Advanced Therapeutics
06.2024 - Current
PhD Research Scholar
Indian Institute of Technology, Hyderabad
07.2018 - 10.2025
Assistant Professor
DCRM Pharmacy College
01.2014 - 06.2018
Ph.D. - Biomedical Engineering
Indian Institute of Technology, Hyderabad
M. Pharmacy - Pharmacology, Pre-clinical Animal Testing
Bapatla College of Pharmacy
B. Pharmacy - Pharmacology, Pharmaceutical Technology, Pharm. Analysis
Vishwa Bharathi College of Pharmaceutical Sciences
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