Suma HK
Bangalore
Summary
A multi-skilled researcher having work experience acquired both in academic and industrial area. Experience with various cross functional teams Quality control, documentation quality assurance and analytical RND. Willingness to adopt new skills and techniques, proactive thinker with good communication and presentation skills, Research project development and project management with proposals and manuscript writing.
Overview
19
19
years of professional experience
Work History
Associate Scientific Manager Analytical RND
Biocon Limited
07.2022 - Current
- Method development and validation for PGI impurities and small molecules using analytical techniques such as HPLC, GC, LC-MS/MS, and GC-MS.
- Preparation and comprehensive review of GC-MS, LC-MS/MS MRM-based quantification method development, validation, method transfer protocols and reports.
- Coordination with Cross Functional teams and third-party labs for Nitrosamine related activity.
- Supporting Regulatory queries, customer queries for API and Peptide projects.
- Supporting in OOS and OOT sample analysis for impurity/unknown peak identification by Q1MS/MS.
Principal Scientist Analytical RND
Biocon Limited
07.2018 - 06.2022
- Method validation for small molecules and peptides using HPLC and LCMS techniques as per ICH guidelines.
- MRM based quantification for peptides (Glatiramer acetate, Liraglutide, Semaglutide, Teduglutide) by using LCMS/MS (AB Sciex QTrap, 6500+) with Analyst Software and Waters XEVO-TQ-S with MassLynx Software.
- Method development for peptides using Circular dichroism spectroscopy (CD) Jasco-1500, Fluorescent spectrometry Jasco-1800.
- Impurity profiling of small molecules by Q1MS/MS.
- Supporting to QC in OOS and OOT sample analysis by Q1MS/MS.
- Supporting to Regulatory queries in impurity identification.
- Reviewing the nitrosamines LCMS based method validation reports.
- Reviewing the method validation and Method transfer Protocol and report preparation, method transfer activities to site QC.
- Giving SOP training to scientist on analytical instruments.
Senior Scientist Analytical RND
Biocon Limited
11.2016 - 06.2018
- Method development and validation for small molecules using HPLC-PDA.
- Involved in method validation and Method transfer protocol and report preparation, method transfer to site QC.
- Routine support to synthetic team by analyzing the API and IPC samples for assay, related substances, reaction monitoring and content by using HPLC.
- Periodic Calibration of instruments HPLC.
- Involved in periodic revision of SOP's for analytical instruments.
Senior Research Fellow
School of Ecology and Conservation lab
02.2009 - 06.2012
- Worked on plant secondary metabolites (Camptothecin, Shikimic acid, Galantamine) using various analytical tools.
- Extraction and Isolation of plant metabolites: Soxhlet and hot water bath were used.
- For identification and purification: TLC, column chromatography, HPLC and Prep HPLC techniques were used.
- Structural characterization of secondary metabolites by using LCMS/MS and NMR.
- Project and drossier report writing to DBT.
Junior Research Fellow in Analytical RND
Avesthagen Ltd
Bangalore
05.2008 - 01.2009
- Worked on plant metabolites by using HPLC for identification of secondary metabolites.
- Metabolites purification by using Preparative HPLC Shimadzu.
- SOP preparation and presenting the data.
Officer-QC Formulation
Medreich Limited
08.2007 - 04.2008
- Worked on Raw material, Bulk and Finished sample analysis using various analytical tools in QC formulation department.
- Preparation of QC analytical instrument SOP and IOP.
- QC lab
Education
PhD - Biochemistry
Davangere University
01.2016
MSc - Biochemistry
Kuvempu University
01.2007
Chemistry, Bio-tech and Botany - undefined
Sahyadri Science College
01.2005
Skills
- Chromatography techniques
- HPLC
- Empower software
- Mass Spectrometry techniques
- AB Sciex LCMS-Qtrap-6500 plus
- Analyst Software
- Waters LCMS-XEVO-TQ-S
- TQD
- MassLynx Software
- Agilent GC-MS
- Mass hunter software
- Protein Characterization techniques
- Capillary Electrophoresis
- Sciex CESI 8000 plus
- Circular dichroism spectroscopy
- CD Jasco-1500
- Fluorescent spectrometry
- Jasco-1800
- Impurity profiling
- Q1MS/MS
- QC support
- OOS sample analysis
- OOT sample analysis
- Regulatory queries
- Impurity identification
- Method validation
- Method transfer Protocol
- Report preparation
- Method transfer activities
- SOP training
- Analytical instruments
Accomplishments
- Selected for Canadian common wealth fellowship in Concordia University, Dept of Biology, Montreal, Canada., 2013-03-01
- Got recognition award from Biocon limited, for working in the field of LCMS/MS for peptide sample analysis.
Publications
- Pyrenacantha volubilis Wight., (Icacinaceae) a rich source of camptothecin and its derivatives, from the Coromandel Coast forests of India., Suma HK, Kumar V, Senthilkumar U, Kumara PM, Ravikanth G, Santhoshkumar TR, Shaanker RU, Fitoterapia, 97, 105-110, 2014-01-01
- Spatial and temporal distribution pattern of camptothecin in seeds and fruits of Pyrenacantha volubilis Hook. (Icacinaceae) during different fruit developmental stages., Suma HK, Kumar V, Kumara PM, Amitava S, Ravikanth G, Pradeep T, Uma Shaanker R., Current Science, 112, 5, 2017-01-01
- New plant sources of the anti-cancer alkaloid, camptothecin from the Icacinaceae taxa, India., Ramesha BT, Suma HK, Senthilkumar U, Ravikanth G, Vasudeva R, Santhosh Kumar TR, Ganeshaiah KN, Uma Shaanker R., Phytomedicine, 20, 521-527, 2013-01-01
- Isolation of endophytic bacteria producing the anti-cancer alkaloid camptothecin from Miquelia dentata Bedd. (Icacinaceae)., Shweta S, Hima Bindu J, Raghua J, Suma HK, Manjunatha BL, Mohana Kumara, Ravikanth G, Nataraja KN, Ganeshaiah KN, Uma Shaanker R., Phytomedicine, 20, 337-342
Presentations
- New plant sources of the anti-cancer alkaloid, camptothecine from the Icacinaceae, India., International conference on Phytochemicals in Health and disease: Challenges and Future opportunities., Department of Biochemistry, Annamalai University, Annamalainagar, 2013-01-23 to 2013-01-25
- Workshop on Natural product isolation and purification, SEC Lab, GKVK, Bangalore, 2012-04-19 to 2012-04-21
Training
- Techniques in Recombinant DNA Technology, JIVAS, Bangalore, 2004-05-01
- Biotechnology Tools and Techniques in Molecular Biology Lab, Central Sericulture Research and Training Institute, Mysore, 2007-06-18 to 2007-07-13
- 1D & 2D NMR Spectroscopy & application in structural Biology, IISC, Bangalore, 2011-02-04 to 2011-05-25
Timeline
Associate Scientific Manager Analytical RND
Biocon Limited
07.2022 - Current
Principal Scientist Analytical RND
Biocon Limited
07.2018 - 06.2022
Senior Scientist Analytical RND
Biocon Limited
11.2016 - 06.2018
Senior Research Fellow
School of Ecology and Conservation lab
02.2009 - 06.2012
Junior Research Fellow in Analytical RND
Avesthagen Ltd
05.2008 - 01.2009
Officer-QC Formulation
Medreich Limited
08.2007 - 04.2008
PhD - Biochemistry
Davangere University
MSc - Biochemistry
Kuvempu University
Chemistry, Bio-tech and Botany - undefined
Sahyadri Science College