Sushovan Dalui
Hyderabad
Summary
Enthusiastic biotech research candidate with strong technical knowledge. Demonstrated strong learning capabilities, zeal and interest in medical biotechnology and applied microbiology. Experienced Research Associate in Pharmaceuticals. Having a good reputation for resolving problems and improving customer satisfaction.
Overview
6
6
years of professional experience
Work History
Research Associate
Aragen Life Sciences
2021.12 - Current
- Doing biochemical assays like HTRF, TR-FRET, Kinase Assay, AlphaScreen, Lance Ultra, Lantha Screen assay.
- Having enough exposure in ELISA
- Mammalian Cell culture along with Cell Based assays like AlphaLisa, CTG assays
- Planned, modified and executed research techniques, procedures and tests according to requirements.
- Analyzing data in Excel sheet (XL fit and Excel) and GraphPad
- Set up equipment, organized inventory and maintained facilities.
Trainee
Biozeen
2021.09 - 2021.10
Attended training courses to build understanding of processes, techniques and industry.
Experienced in Chromatographic Techniques, Tangential Flow Filtration, Ultrafiltration, Microfiltration, SDS PAGE. Used bacterial liquid cultures to filter out amylase. Later filtrates and concentrates were used for purification of amylase using Ion exchange chromatography. Colour change in DNS assay preliminarily confirms presence of amylase, and its activity was calculated. SDS PAGE was used to confirm the purification.
Summer Intern
Jadavpur University
2018.06 - 2018.08
Silver nanoparticles were produced from food waste (Litchi seeds and Skins) and were tested for its application in production of antibiotics. Aqueous and methanolic extract was used for food waste sample. Silver nanoparticles were prepared using bottom up approach and optimized the conditions (Temp and amount of extract). Then particles were characterized and tested using SEM, TEM, FTIR for antibiotic production.
Summer Intern
West Bengal University of Animal and Fishery Science
2017.07 - 2017.08
Rabbit and Goat Serum samples were tested for reaction, to produce Rabbit anti-goat IgG Secondary antibody for further testing. Serum antibody extraction kit was used to extract antibody from serum samples. Then protein A chromatography was done to purify the extracted antibody. A simple radial immunodiffusion assay was done to test the interaction of antibody antigen.
Education
M.Tech - Biotechnology
Indian Institute of Technology Guwahati
Guwahati, Assam09.2021
B.Tech - Biotechnology
Haldia Institute of Technology
Haldia, West Bengal07.2019
Higher Secodary - Sceince
WBCHSE
West Bengal05.2015
Secondary - General Studies
WBBSE
West Bengal05.2013
Skills
Molecular Biology Techniques: SDS PAGE; Gel Electrophoresis; PCR; DNA Isolation; RNA Isolation; Gel Preparation; Spectrophotometry; Chromatography; ELISA
Microbiological Techniques: Media Preparation; Colony Isolation; Aseptic
Culturing; Gram Staining; Microscopy; Sterilization;
Biochemical Assay: HTRF; TR-FRET; Kinase Assay; Alphascreen; lance Ultra; Ubiquitinatrion
Downstream Methods: Chromatographic Techniques; Tangential Flow Filtration; Ultrafiltration; Microfiltration
Equipment Handling: Liquid Handlers (Echo and Biomek), Multi Plate Readers (Envision, Spectramax).
Certifications
• Attended a National Seminar on “New Horizons in Biotechnology” poster presentation ‘Bio Toilet’ Feb, 2017 Dept of Biotechnology, Haldia Institute of Technology.
• Certified from IIT Kharagpur Nanotechnology for Healthcare, organized by GIAN
• Certified from IIT Kharagpur for NPTEL Course on Soft Nanotechnology, Nanotech in Agriculture, Industrial Biotech
• Participated in “Advance C” training offered by the spoken tutorial project, IIT Bombay, 2017
• Attended the webinar on ‘Groundwater Security of India’ delivered by Dr. Abhijit Mukherjee (Shanti Swarup Bhatnagar Awardee, 2020), Associate Professor, IIT Kharagpur
• Participated in the Shastri Indo-Canadian Institute sponsored Indo-Canada online workshop on NanoBioengineering jointly organized by the Department of Biotechnology, Indian Institute of Technology (IIT) Roorkee and Centre for Biomedical Research (CBR), University of Victoria (UVic) Canada
Notable Projects
Enzymatic Remediation of Pesticides contaminated run-off in paddy
fields: A Holistic approach
Degradation of pesticides in wastewater by various enzymes involved in the
Advanced Oxidation Process (AOP) : Oct 2020
Detection of Arsenic from Biologically Synthesized Nanoparticle
: May 2021
Detection of Arsenic (III) in wastewater using Biosynthesized PbS and Ag nanoparticles
Green Synthesis of Silver Nanoparticles using Litchi : Jun 2018
Synthesis of silver nanoparticles from waste material for antibiotics.
Adsorption of Chlorpyrifos by Potato Peel Biochar : Sep 2018
Characterization of Organophosphate Pesticide Sorption of Potato Peel Biochar as
Low-Cost Adsorbent for Chlorpyrifos (Published in Chemosphere Journal in 2022 , DOI:
doi.org/10.1016/j.chemosphere.2022.134112 )
Timeline
Research Associate
Aragen Life Sciences
2021.12 - Current
Trainee
Biozeen
2021.09 - 2021.10
Summer Intern
Jadavpur University
2018.06 - 2018.08
Summer Intern
West Bengal University of Animal and Fishery Science
2017.07 - 2017.08
M.Tech - Biotechnology
Indian Institute of Technology Guwahati
B.Tech - Biotechnology
Haldia Institute of Technology
Higher Secodary - Sceince
WBCHSE
Secondary - General Studies
WBBSE
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