Sweatha R

• Media preparation & Sterilization of glassware's and tools required for culture work.
• Maintenance and Subculture of plant hairy roots in solid and Liquid medium.
• Carried out different experiments using cultures grown in bulk in 250ml Conical flask to 15L temporary bioreactors.
• Collection of samples in sterile condition for extraction and preparation of samples for GC-MS and HPLC periodically.
• Following standard operating procedures,regular inspection,cleaning & reporting any problems or defects.
• In addition to source quotations for consumables & instruments with the comparative statements for the best purchase option. This involves contacting various suppliers,comparing the costs and quality and negotiating the best offers.
• To ensure smooth and consistent supply of consumable's,I perform monthly stock maintenance. This involves checking inventory levels,ordering new items as required.
• Maintenance of safe and clean laboratory environment, adhering to safety guidelines and promptly addressing any hazards.
• Conducted routine equipment maintenance to prevent downtime and ensure optimal functioning of instruments.
• Trained new Laboratory Technologists on best practices and standard operating procedures, ensuring consistent team performance.

• Media preparation & Sterilization of glassware's and tools required for culture work.
• Plant collection,Surface sterilization ,inoculation of explants in solid medium for regeneration of rare endangered species.(plant tissue culture)
• Plant sample collection, drying & fresh and dried sample carried out for extraction process such as homogenization,serial exhaustive extraction,soxhlet extraction,Decoction based on plant material.
• Qualitative and Quantitative analysis of plant samples.
• Basic molecular biology techniques like DNA extraction and PCR amplification was carried out for few other experiments.
• Following standard operating procedures,regular inspection,cleaning & reporting any problems or defects.
• Maintenance of a safe and clean laboratory environment, adhering to safety guidelines and promptly addressing any hazards.

My role as Junior executive:

In Plant tissue culture :

• Media and stock preparation ,sterilization of glassware's and tools required for culture work.
• Callus culture,leaf culture,shoot and root induction,primary hardening.

In Phytochemistry:

• Extraction process such as homogenization,serial exhaustive extraction,soxhlet extraction,Decoction based on plant material.
• Preliminary screening of phytocomponents.
• Antioxidant activity of phytocompounds.

In Microbiology:

• Sterilization,Media Preparation,Microbial culture methods,Isolation of bacteria from soil and water,Staining technique,Antimicrobial activity.

In Molecular biology:

• Basic molecular biology techniques such as DNA extraction and PCR amplification was carried out for few other experiments.
• Conducted extensive literature reviews, summarizing key findings to inform future studies.
• Improved research quality by implementing rigorous data collection methods and analytical techniques.
• Recorded and analyzed data to produce reports of results.
• Trained and supervised Students who came for projects in laboratory protocols.