Varad Arkatkar

• Performed PCR, DNA sequencing, and other molecular biology techniques to analyze gene expression.
• Conducted literature reviews to identify potential areas for research.
• Analyzed data using statistical software packages, such as R.
• Operated sophisticated lab equipment, such as spectrophotometers, PCR machines, and sequencers.
• Maintained accurate records of all experimental work performed in notebooks or electronic databases.
• Provided technical support to colleagues within the laboratory environment by troubleshooting instruments or helping them understand complex concepts related to biotechnology.
• Used bioinformatics applications in my project

My undergraduate project was an insightful experience, as it allowed me to learn the techniques of molecular cloning. I cloned the CBM 32 domain of a gene which encodes for beta-galactosidase, an enzyme capable of breaking down complex carbohydrates, revealing its remarkable versatility in carbohydrate binding. I incorporated that gene by ligating it inside pET-28a (+) vector and transforming the vector in E.coli Top 10 system. The diversity within the CBM family 32 suggested a broader role for these modules in various carbohydrate-processing enzymes across different organisms. Through this project, I not only honed my technical skills in molecular biology but also developed appreciation for the complexity and adaptability of biological systems.