Varun Koli

• Development of applications using GC, GC-HS (Both Loop and Trap mode) and GC-MS/MS techniques in various multi-disciplinary areas.
• Significant experience of MRM optimization, multi-residue pesticide analysis from various complex matrices and trained in working with detectors like FID, TCD, ECD, FPD, FTD and BID.
• Improvisation in sample preparation, pretreatment, clean-up strategies, method development and part method validation for research areas like food safety, clinical, pharmaceuticals, etc.
• Data processing, evaluation and interpretation to draw valid inferences.
• Appropriate use of various sample introduction devices, columns and detectors as per need of application.
• Address troubleshooting issues by taking suitable measures based on logical approach.
• Conduct scheduled trainings, customized on-site trainings, method transfers for various instruments for pre-sales evaluations and provide post-sales support to clients.

• Method development for various R & D - API Projects for Impurity Profiling
• Method development for chiral as well as Achiral separations in both modes of HPLC
• Analyzing External customer’s samples as per IP/BP/USP
• Analyzing External customer’s sample for mass identification up to 0.1 % level by LCMS
• Analyzing Sample by Direct Infusion method on LCMS
• Method Development and Purification of internal samples using preparative HPLC; Transfer of HPLC method to preparative for purification of samples
• Analysis of commercial solvents useful for synthesis by performing various tests by Gas Chromatography
• Performing Calibration of instruments, Maintenance and Troubleshooting of instruments as and when required

Instrument handled:

• HPLC: Waters 2695 (Empower) with PDA, ELSD and RI detectors,
• Agilent 1200 Series (Ezchromlite)
• Shimadzu (LC Solution)
• GC: Agilent 7820 (EzChromlite)
• LC-MS: Waters 2695 (Masslynx) with PDA/ESI/APCI mode
• Thermo (Xcalibur, LCQ Tune) with ESI and Ion trap analyzer
• UPLC-MS: Waters Acquity (Masslynx) with PDA/ESI/APCI mode.