VIGNESH VENKATESH IYER

Highly motivated and detail-oriented Biotechnology graduate currently pursuing a Master's in Biotechnology at Nottingham Trent University, with substantial hands-on experience in molecular and cell biology techniques. Proficient in cell culture, PCR, qPCR, gel electrophoresis, and Western blotting, complemented by experience in microbial diagnostics and genomics workflows. Conducted advanced research project on the role of carnosine in modulating inflammation in immune cells ,demonstrating strong experimental design and data analysis skills. Known for adaptability, precision, and a collaborative mindset, eager to contribute to innovative R&D projects, particularly in the areas of cell rejuvenation and disease research.

Molecular Biology Techniques

· Polymerase Chain Reaction (PCR)

· Quantitative PCR (qPCR)

· Plasmid Transformation

· Agarose Gel Electrophoresis (AGE)

· BCA assay

· SDS-PAGE

· Tissue culture using THP-1 Cells

· Hydrophobic Interaction Chromatography

· Western Blotting

Microbiology Techniques

· Microbial Isolation and Identification

· Disk Diffusion

· Minimum Inhibitory Concentration (MIC) Assay

· API Testing

· Latex Agglutination Assay

Additional Techniques

· MTT Assay

· Chromatin Immunoprecipitation (ChIP)

Cell Counting and Analysis

· Counting of RBC and WBC using a Haemocytometer

· Differential Count of WBC

· Estimation of Haemoglobin

· ABO Blood Grouping

Cell Division Studies

· Mitotic Division of Onion Root Tip

Enzyme Activity Studies

· Effect of pH and Temperature on the Activity of Salivary Amylase

· Survey of Digestive Enzymes in Cockroach

Estimation Studies

· Oxygen in Fish with Reference to Body Weight

· Dissolved Oxygen and Carbon Dioxide in Water Samples

· Carbonate and Bicarbonate in Water

· Salinity in Water Samples

Dissection Studies

· Cockroach: Digestive System, Nervous System, Reproductive System

· Fish: Digestive System, Urogenital System

Microscopy and Mounting

· Mounting of Cockroach Mouthparts

Qualitative Analysis

· Carbohydrates

· Amino Acids

· Excretory Products

Ciliary Activity

· Ciliary Activity in Freshwater Mussel

Analysis

· Zooplanktons

• Certificate Course in Industrial Biotechnology by the University of Manchester.
• Workshop on Cancer Biology - Participated in a workshop on the topic of Cancer Biology at the Indian Institute of Science (IISc), Bangalore.
• Seminar on Vaccine Technology - Attended a seminar on the topic of Vaccine Technology at the Indian Institute of Science (IISc), Bangalore.
• Guinness World Record - Largest Biology Class - Participated in the Largest Biology Class, setting a Guinness World Record.

• Laboratory Techniques: I have developed a strong interest in performing various laboratory techniques, particularly: Tissue Culture, BCA Assay, SDS-PAGE and Western Blotting. These techniques are integral to my research project, and I excel at them through repeated practice. I prefer practical learning over theoretical learning, which allows me to master these complex procedures.
• Travel and Photography: Exploring new places and capturing moments through photography and videography is one of my passions. I enjoy editing these visual stories in a way that captivates viewers on social media. This hobby not only satisfies my creative side but also hones my technical skills in photo and video editing.
• Sports Enthusiast: In my free time, I am an avid sports enthusiast. I particularly enjoy watching cricket and football. Following these sports keeps me entertained and allows me to relax and unwind.