Adityo Paul

I am an passionate downstream protein purification specialist currently looking for opportunities to expand my horizon to gain knowledge and novel skills to explore and develop innovative techniques that can revolutionize the biopharmaceutical industry and biomedical technology.

University of Glasgow | Oct 2015 to Aug 2017

1. Successfully over-expressed the mature gene sequence for recombinant human MsrB3A protein into recombinant E. coli cells to generate sufficient protein for further structural and functional studies.

2. Recombinant protein was successfully extracted using French Press and purified via column chromatography on AKTA purification systems.

3. Structural analysis and studies of the purified protein were conducted using CD spectroscopy, NMR spectroscopy, and crystallography.

4. Non-functional MsrB3A protein was made using sequence analysis, sequence alignment and site-directed mutagenesis. The gene sequence was then efficiently cloned and further over-expression in recombinant E. coli cells.

5. Functional studies were conducted using a protein-based Gel Shift Assay and Reverse-Phased Chromatographic assay to prove protein functionality and stereospecificity.

Heritage Institute of Technology | Jul 2013 to Jun 2014

1. Collected and processed matured Tinospora cordifolia plant stems to obtain crude enzyme extract and further concentrated using lyophilization.

2. Estimated concentration of amylase enzyme in the enzyme extract by specific activity.

3. Prepared standard estimation curves for reducing sugar and lactic acid concentrations.

4. Sub-cultured Lactobacillus spp. consortium for further fermentation studies.

5. Conducted lactic acid fermentation studies using different concentrations of glucose, and jaggery as energy sources at different time intervals.

6. Conducted the simultaneous saccharification and lactic acid fermentation using corn starch as energy source.

• Radiation protection course | University of Glasgow | 29th October 2015.
• In-plant summer training | Bio-Caps India Limited | 15th June 2013 to 11th July 2013.
• In-plant summer training (99.9% alcohol production plant) | IFB Agro Industries Limited | 13th June 2011 to 28th June 2011.

• Have participated in the international "Advances in Healthcare Engineering" workshop at the College of Engineering and Management, Kolaghat.
• Attended seminar on "Biopolymers in Biomedical Engineering" organized by the School of Biosciences and Engineering, Jadavpur University, Kolkata.
• Participated in the essay competition on Germplasm Bank for a seminar on "Food Security and GM Crops" organized by the Department of Biotechnology, Heritage Institute of Technology, Kolkata.
• Participated in the seminar on "Observation of Energy Conservation Day 2011 and 4th Renewable Energy & Energy Efficiency Business Forum" organized by the Indian Institute of Social Welfare and Business Management.

1. Amateur Photographer mostly specializing in candid photography.

2. Novice pianist having Grade 1 certification from Trinity College London.

3. Long endurance cycling enthusiast.

4. Novice football (soccer), table tennis, and video-game enthusiast.

1. Dr. Arnish Chakraborty, Senior Research Scientist, TCG Lifesciences Private Limited (arnish.chakraborty@gmail.com / arnish.chakraborty@tcgls.com)

2. Mr. Sameer Hadawale, Downstream Purification Specialist, UCB Belgium (sameer.hadawale4@gmail.com / sameer.hadawale@ucb.com)

3. Dr. Riddhi Goswami, Associate Professor and Department Coordinator, Heritage Institute of Technology (rgoswami77@gmail.com / riddhi.goswami@gmail.com)

4. Mr Krishan Kant Srivastava, Research Scientist, Syngene International Limited (Krishan.Kant@syngeneintl.com / kant.krishan87@gmail.com)