Aishwarya Patare
Executive (R&D)
Pune
Summary
Dedicated Research and Development Executive with 3 years of experience. Successful at managing multiple priorities with positive attitude and willing to take on greater responsibilities to meet team goals. Carrying out thorough research, analysis and evaluation for the most accurate results, confidently reporting and presenting findings to best meet business needs.
Overview
6
6
years of professional experience
5
5
years of post-secondary education
3
3
Certifications
3
3
Languages
Work History
Research and Development Executive
Gennova Biopharmaceuticals Vaccine Formulation and Research Center
Pune, India
04.2021 - Current
- Design and development of antigenic cassette of mRNA vaccine for COVID-19 – (Vaccine commercialized under the brand name GEMCOVAC-OM).
- Production of pseudovirus and performed the pseudo-virus neutralization of clinical samples of GEMCOVAC-19.
- Designed and cloned novel chimeric antigen receptors for CAR T cell therapy in the mRNA platform and in a lentivirus platform.
- Manufactured CAR-expressing mRNA and lentivirus of the designed candidates and developed in-vitro cellular assays for CAR characterization.
- Planned research activities to develop Next-generation mRNA production process to minimize the manpower, reagent, facility utilization, and analytics.
Research Trainee
Patare, National Centre for Microbial
Pune
12.2019 - 03.2020
- Worked on a project titled 'Incidence of Antimicrobial Resistant bacteria encountered on the surface of vegetables and fruits'
- It involved the isolation of microbes from the surface of vegetables and fruits using microbiological techniques- spread plate technique
- Establishing pure cultures-streak plate method Cryopreservation
- DNA extraction, PCR, PEG purification rRNA sequencing and MALDI-TOF -identification of organisms up to species level
- Antimicrobial assay
Biology teacher
Agate Classes
Pune, India
08.2018 - 09.2019
- Motivated students by demonstrating deep subject knowledge and passion for scientific practice.
Education
Master of Science - Biotechnology
Vidya Pratisthans' School of Biotechnology
India 06.2018 - 04.2020
Bachelor of Science - Biotechnology
Vidya Pratisthans' School of Biotechnology
India 06.2015 - 04.2018
Skills
DIGITAL SKILLS
Microsoft Word / Microsoft Excel / Microsoft Powerpoint
OTHER SKILLSGood listener and communicator / Team-work oriented / Organizational and planningTECHNICAL SKILLSCloning and mutagenesis of the genes
Cloning using Gibson assembly, designing primers for cloning and sequencing, PCRDesigning Primers for cloning and sequencing
Conventional PCR and Quantitative Real-Time PCR
Mammalian cell culture and transfection of plasmids for expression of proteins in mammalian cells
Pseudovirus Preparation and Neutralization assays
IVT mRNA preparation and cDNA synthesis
Quantitative analysis of DNA and mRNA
Agarose Gel Electrophoresis
Sanger and Next generation sequencing
FACS assay and analysis
Accomplishments
- Evaluation of self-amplifying mRNA platform for protein expression and genetic stability : Implication for mRNA therapies submitted to BBRC, Elsevier group.
- Thermostable self -amplifying mRNA vaccine GEMCOVAC-OM manuscript under consideration at Nature Biotechnology.
- In-house assembled Omicron spike gene sequence submitted to DDBJ database having accession ID-LC731729.
Certification
Awarded First in National level Poster Presentation competition of Immunofest held at VPASCC.
Hobbies
Listening to music, singing, craftwork, baking
Timeline
Awarded First in National level Poster Presentation competition of Immunofest held at VPASCC.
09-2024
Research and Development Executive
Gennova Biopharmaceuticals Vaccine Formulation and Research Center
04.2021 - Current
Research Trainee
Patare, National Centre for Microbial
12.2019 - 03.2020
Participated in two days National Level workshop on Chromatographic and Spectroscopic Findings in Life Science Research organized by VPASCC.
08-2019
Certified for participating in 104 th Indian Science Congress held at S.V University, Tirupati.
01-2019
Biology teacher
Agate Classes
08.2018 - 09.2019
Master of Science - Biotechnology
Vidya Pratisthans' School of Biotechnology
06.2018 - 04.2020
Bachelor of Science - Biotechnology
Vidya Pratisthans' School of Biotechnology
06.2015 - 04.2018