Ankit Kumar
Chandigarh
Summary
Accomplished Senior Research Fellow at PGIMER, Chandigarh, with extensive experience in host pathogen interaction, immunoepigenomics, molecular diagnostics and assay development. Demonstrated expertise in sample collection, processing, and execution of advanced molecular techniques such as real-time PCR and LAMP assays. Proven ability to analyze experimental data, maintain meticulous documentation, and ensure quality control. Skilled in DNA isolation, protein isolation, mammalian cell culture, ELISA, recombinant cloning, and bioinformatics. Adept at collaborating with multidisciplinary teams to achieve project goals. Career goal: To leverage extensive research experience to contribute to innovative advancements in molecular diagnostics and infectious disease research.
Overview
7
7
years of professional experience
Work History
Senior Research Fellow
Post Graduate Institute of Medical Education and Research
Chandigarh
04.2024 - Current
- Sample Collection and Processing: Collecting clinical samples from leprosy patients and processing them for downstream molecular analyses.
- Assay Development and Standardization: Developing and optimizing molecular assays, such as real-time PCR, for the detection of M. leprae DNA.
- Molecular Techniques Execution: Performing molecular diagnostic tests, including conventional PCR, real-time PCR, and LAMP assays, for evaluating assay sensitivity and specificity.
- Data Analysis and Interpretation: Analyzing experimental results to assess assay performance and interpreting data for further refinement.
- Documentation and Reporting: Maintaining accurate records of experiments and preparing detailed reports for project updates and publication.
- Collaboration and Communication: Coordinating with team members and contributing to scientific discussions to advance the project goals.
- Quality Control: Ensuring reproducibility and reliability of results through stringent quality checks and protocol adherence.
- Secured funding through grant writing for ongoing and future research projects.
- Supported doctoral candidates with molecular cloning techniques for their dissertations.
Junior Research Fellow
Postgraduate institute of Medical Education and Research
Chandigarh
10.2017 - 07.2018
- Contributed to research on immunodominant B cell epitopes of secretory RD antigens in Mycobacterium tuberculosis.
- Executed tasks related to sample collection and processing.
- Utilized computational methods to detect immunodominant epitopes.
- Conducted ELISA testing and data analysis.
- Maintained laboratory equipment, guaranteeing optimal performance for experimental procedures.
- Utilised various methods of research to obtain material for examination, including case, ethnographic, survey and experimental research.
- Coordinated with senior researchers to design research methodologies, ensuring adherence to project objectives.
Education
Ph.D. - Biochemistry
Postgraduate Institute of Medical Education And Research
Chandigarh, India07.2024
M.Sc. - Molecular biology and Biochemistry
Guru Nanak Dev University
Amritsar, India05.2017
Bachelor of science - Biochemistry
Kurukshetra University
Kurukshetra, India01.2015
Skills
- Molecular and Cellular Biology
- DNA isolation
- Protein isolation
- Restriction fragment length polymorphism
- Cell culture
- ELISA
- PCR
- SDS-PAGE
- Recombinant Cloning
- FACS
- COBRA assay
- Primary immune cell isolation
- Mycobacterial culture handling
- Media preparation
- Staining
- Bioinformatics
- Gene set enrichment analysis
- Whole genome bisulfite sequencing data analysis
- Sanger sequencing data analysis
- MS-Office
Accomplishments
- CSIR -UGC JRF (June 2017) qualified national eligibility test (NET) and got Union grant commission fellowship as junior research fellow
- CSIR -UGC JRF (Dec. 2017) qualified national eligibility test (NET) and qualified as a CSIR-JRF.
- Travel grant funded by The lung Union for attending The lung union conference on Lung health 2024
Publications
- Ankit Kumar, Dr. Jyotdeep Kaur, Dr. Ashutosh Nath Aggarwal, Dr. Indu Verma, TB Immunology and pathogenesis, Textbook of Pulmonary and Critical care medicine, 3rd edition, 2024
- Ankit Kumar, Dr. Jyotdeep Kaur, Dr. Ashutosh Nath Aggarwal, Dr. Indu VermaThe altered differential genome-wide DNA methylation profile of peripheral blood mononuclear cells in pulmonary tuberculosis. Indian Journal of Microbiology, 63, Proceedings of 63rd annual international association of Microbiologists of India, 408, 2023
- Ankit Kumar, Dr. Sumedha Sharma, Dr. Jyotdeep Kaur, Dr. Ashutosh Nath Aggarwal, Dr. Indu Verma .Identification of proteomic biomarkers in Pulmonary TB with a possible role in host methylome modification, Indian Journal of Clinical Biochemistry, 37, 1 - 152, 2022,
- Manoj Kumar, Roop Laal, Amit Sehgal, Ankit Kumar, Santosh Kumar, Sunil dhatwalia, Green Tea's EGCG: Brewing hope in the battle against Breast cancer. The Natural Products Journal, Bentham science publishers, accepted and to be published online by January 2025
Training, Confrences and workshops
- Attended TBCON 2019 at PGIMER, Chandigarh, India
- Attended workshop on BSL-3 safety and microbial investigation organized by department of medical microbiology, PGIMER, Chandigarh, India
- Attended symposium on theme “Towards End TB: Achievements, Challenges and Future Directions.”
- e-Poster Presentation in NZACBICON 2022
- Attended and poster presentation 48th ACBICON 2023, New Delhi, India on theme “Harnessing Basic and molecular research to enhance patient care.”
- Attended and presented poster in 61stAMICON 2021, New Delhi, India on theme “Microbial world: Recent development in health, Agriculture and Environmental science.”
- Attended and presented poster in 63rd Annual International Conference of AMI 2023, Rohtak, India on theme “ Microbial technologies for sustainable biosphere.”
- e-Poster presentation in The Lung Union Conference on Lung health 2024
- e-Poster presentation in ACBICON 2024
Timeline
Senior Research Fellow
Post Graduate Institute of Medical Education and Research
04.2024 - Current
Junior Research Fellow
Postgraduate institute of Medical Education and Research
10.2017 - 07.2018
Ph.D. - Biochemistry
Postgraduate Institute of Medical Education And Research
M.Sc. - Molecular biology and Biochemistry
Guru Nanak Dev University
Bachelor of science - Biochemistry
Kurukshetra University
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