Post Doctoral Research Associate
Iowa state University
Ames
05.2024 - Current
Cell Culture:
- Hands-on experience with HepG2 cell culture (uninfected and parasite-infected cells)
- Hybridoma cell culture (aseptic techniques, maintenance, subculture, cryopreservation)
- Drug screening in infected HepG2 cell culture
- Cell cytotoxicity assay
- Proficient in maintaining sterile conditions to prevent contamination
- Experienced in the optimization of culture conditions for cell growth and proliferation
- Skilled in cryopreserving cells and ensuring long-term viability
Genomics:
- Expertise in designing CRISPR/Cas9 strategies for gene editing
- Southern blotting and transcriptomics
- Primer designing, genomic DNA isolation, and DNA cloning
- RNA isolation, cDNA synthesis, and quantitative PCR.
- Proficient in troubleshooting and optimizing PCR conditions
- Experienced in performing transcriptome analysis to study gene expression patterns
Proteomics:
- Recombinant protein expression and purification
- SDS-PAGE and Western blotting
- Immuno-pulldown assay and antibody generation
- Skilled in optimizing protein expression conditions for maximum yield
- Proficient in protein purification techniques, including affinity chromatography
- Experienced in interpreting Western blot results and troubleshooting issues
Imaging Techniques:
- Immunofluorescence assay
- Extensive use of bright field and fluorescence microscopy
- Familiarity with commonly used fluorophores
- Skilled in preparing samples for microscopy, including fixation and staining
- Proficient in using image analysis software to quantify fluorescence intensity
- Experienced in multi-channel imaging and co-localization studies
Electroporation method for transfection:
- Transfection in Plasmodium-infected RBCs
- Parasite cloning to obtain clonal populations by limiting dilution
- Expertise in electroporation methods
- Skilled in optimizing transfection efficiency and minimizing cell toxicity
- Experienced in selecting and expanding successfully transfected cell populations
Assays and Techniques:
- Proficient in performing cell viability and proliferation assays
- Experienced in conducting cytotoxicity assays to evaluate drug effects
- Skilled in setting up and interpreting results from various bioassays
Data Analysis:
- Experienced in using bioinformatics tools for sequence alignment and analysis
- Proficient in statistical analysis using GraphPad Prism
- Skilled in presenting data through clear and concise figures and tables
Additional Skills:
- Strong background in molecular cloning techniques
- Proficient in performing immunoprecipitation and co-immunoprecipitation assays
- Experienced in conducting functional assays to study protein activity
