Dani Lakshman Yarlagadda

Professional Summary

I am an exceptionally focused and dedicated researcher with an outstanding work ethics. I am committed to advancing knowledge and innovation in drug development and pharmaceutical research. As a researcher with strong foundation in drug metabolism and pharmacokinetics, analytical and bioanalytical method development, I have consistently contributed to cutting-edge projects aimed at improving healthcare outcomes. My research journey has been characterized by a passion for drug discovery, metabolism, dose optimisation and pharmacokinetics. I am seeking a challenging position in a professional organization where I can utilize my skills and abilities to meet its goals and provide outstanding research contributions.

• Dani Lakshman Yarlagadda l LinkedIn https://www.linkedin.com/in/dani-lakshman-yarlagadda-a61346152/
• Dani Lakshman Yarlagadda l ORCiD https://orcid.org/my-orcid?orcid=0000-0002-4749-4162

1. Yarlagadda DL, Anand VS, Nair AR, Dengale SJ, Pandiyan S, Mehta CH, Manandhar S, Nayak UY, Bhat K. A computational-based approach to fabricate Ceritinib co-amorphous system using a novel co-former Rutin for bioavailability enhancement. European Journal of Pharmaceutics and Biopharmaceutics. 2023 Sep 1;190:220-30. https://doi.org/10.1016/j.ejpb.2023.07.019 

2. Yarlagadda DL, Nayak AM, Brahmam B, Bhat K. Exploring the Solubility and Bioavailability of Sodium Salt and Its Free Acid Solid Dispersions of Dolutegravir. Advances in Pharmacological and Pharmaceutical Sciences. 2023 Jun 20;2023. https://doi.org/10.1155/2023/7198674

3. Yarlagadda DL, Nayak AM, Bhat K. Solid State Characterization and Miscibility of Raltegravir in Soluplus Using Solid Dispersion Technology. Indian Journal of Pharmaceutical Education & Research. 2023 Jul 2;57. https://doi.org/10.5530/ijper.57.3s.63

4. Yarlagadda DL, Anand VS, Nair AR, Sree KN, Dengale SJ, Bhat K. Considerations for the Selection of Co-formers in the Preparation of Co-amorphous Formulations. International Journal of Pharmaceutics. 2021 Apr 27:120649. https://doi.org/10.1016/j.ijpharm.2021.120649.

5. Yarlagadda DL, Chegireddy M, Hanegave GK, Sree KN, Kumar N, Lewis SA, Dengale SJ. Investigation of drug-polymer miscibility, biorelevant dissolution, and bioavailability improvement of Dolutegravir-polyvinyl caprolactam-polyvinyl acetate-polyethylene glycol graft copolymer solid dispersions. European Journal of Pharmaceutical Sciences. 2020 Jan15;142:105137. https://doi.org/10.1016/j.ejps.2019.105137. 

6. Perdalkar S, Yarlagadda DL, Pai CG, Shetty S, Bhat K. Liquid chromatographic methods in the determination of inosine monophosphate dehydrogenase enzyme activity: a review. Bioanalysis. 2022 Nov;14(22):1453-70. https://doi.org/10.4155/bio-2022-0212

7. Shetty D, Yarlagadda DL, Brahmam B, Dengale SJ, Lewis SA. Investigating the influence of the type of polymer on sustaining the supersaturation from amorphous solid dispersions of Apremilast and its pharmacokinetics. Journal of Drug Delivery Science and Technology. 2023 Jun 1;84:104520. https://doi.org/10.1016/j.jddst.2023.104520 

8. Pisay M, Yarlagadda DL, Vullendula SK, Bhat K, Kunnatur Balasundara K, Mutalik S. Effervescence-induced amorphous solid dispersions with improved drug solubility and dissolution. Pharmaceutical Development and Technology. 2023 Feb 7;28(2):176-89. https://doi.org/10.1080/10837450.2023.2172039 

9. Vullendula SK, Nair AR, Yarlagadda DL, Sree KN, Bhat K, Dengale SJ. Polymeric solid dispersion Vs co-amorphous technology: A critical comparison. Journal of Drug Delivery Science and Technology. 2022 Nov 12:103980. https://doi.org/10.1016/j.jddst.2022.103980. 

10. Uppala S, Vullendula SK, Yarlagadda DL, Dengale SJ. Exploring the utility of co-amorphous materials to concurrently improve the solubility and permeability of Fexofenadine. Journal of Drug Delivery Science and Technology. 2022 Jun 1;72:103431. https://doi.org/10.1016/j.jddst.2022.103431. 

11. Shete S, Reddy SC, Yarlagadda DL, Vullendula SK, Mehta CH, Nayak UY, Dengale S. Implications of phase solubility/miscibility and drug-rich phase formation on the performance of co-amorphous materials: The case of Darunavir co-amorphous materials with Ritonavir and Indomethacin as co-formers. International Journal of Pharmaceutics.2021Oct25;608:121119.https://doi.org/10.1016/j.ijpharm.2021.121119.

12. Nair AR, Yarlagadda DL, Anand VS, Sree KN, Bhat K, Dengale SJ. Overview of Extensively Employed Polymeric Carriers in Solid Dispersion Technology. AAPS PharmSciTech. 2020 Nov;21(8):1-20. https://doi.org/10.1208/s12249-020-01849-z.

13. Muvva A, Yarlagadda DL, Dwibhashyam VM, Dengale S, Lewis SA. In vitro-in silico evaluation of Apremilast solid dispersions prepared via Corotating Twin Screw Extruder. Journal of Drug Delivery Science and Technology. 2020 Oct 1;59:101844. https://doi.org/10.1016/j.jddst.2020.101844.

14. Chegireddy, M., Hanegave, G.K., Yarlagadda DL., Urazov, A., Sree, K.N., Lewis, S.A. and Dengale, S.J., 2020. The significance of utilizing in vitro transfer model and media selection to study the dissolution performance of weak ionizable bases: investigation using saquinavir as a model drug. AAPS PharmSciTech, 21(2), pp.1-14. https://doi.org/10.1208/s12249-019-1563-0.

• Another Ten publications are in the pipeline.

Certificate Course in Biostatistics, Epidemiology and Research Methodology, Manipal College of Pharmaceutical Sciences

Instruments:

• Highly experienced in handling instruments including Thermo LTQ XL Linear Ion Trap Mass Spectrometer, Waters 2695 Separation module HPLC, Shimadzu prominence UHPLC.
• Analytical/Bioanalytical method development & validation by using HPLC and LC-MS/MS using different ionization techniques such as Electrospray Ionization (ESI), Atmospheric Pressure Chemical Ionization (APCI).
• UV-Visible Spectrophotometer, FT-IR, Spectrofluorometer
• BD accuri c6 flow cytometer

Softwares:

• Instrumental Software:  XCalibur software, Empower 3, LC solutions.

• Data Analysis Software: R software, Graphpad, Origin, Microsoft excel.

In-Vitro And In Vivo:
• Bioanalytical method development and validation (Plasma and Tissues)
• Well-experienced in conducting analytical DMPK activities like in vitro plasma stability, transporter inhibition and  drug-drug/herb interaction studies.
• Animal handling in Pharmacokinetics and biodistribution studies
• Cytotoxicity, cell cycle and cell death assays.

DOCTORAL RESEARCH |  Manipal College of Pharmaceutical sciences, MAHE, Manipal | 2019-Present 

Title: “ Fabrication of Ceritinib Binary co-amorphous systems with Plausible Improved Solubility and Bioavailbility. ” 

Currently, I am pursuing my Ph.D. under Prof. Krishnamurthy Bhat  at,  Manipal College of Pharmaceutical sciences, MAHE, Manipal. 

During my Ph.D., I have worked on the preparartion of co-amorphous systems of Ceritinib with phytomolecules like Rutin and Naringin, which were selected by computational method. The prepared amorphous systesms were characterised using DSC, PXRD and FTIR for homogenous amorphous phase and inter molecular interactions confirmation.  The in vitro solubility, supersaturation potential were studied for solubility improvement. Further, the ex-vivo permeability and role of p-gp efflux transporter were studied using everted gut sac method for the co-amorphous systems. Finally in vivo pharmacokinetics of co-amorphous systems were performed on male wistar rats to examine the translation of in vitro solubility in in vivo bioavaibility using UHPLC. I have also evaluated the co-amorphous systems cytotoxicity, cell death and cell cycle analysis by using SRB assay, AOEB staining and Flow cytometry. 

Projects handled 

• Method Development And Validation For Simultaneous Estimation Of Glycyrrhizin, Tannic Acid And Piperine By RP-HPLC.
• Method Development And Validation (Plasma and Brain) for Estimation of N acetyl L Tryptophan in the Aβ 1-42-induced neuroinflammation using RP-HPLC.
• Method Development And Validation (Brain and plasma) for Estimation of Temozolomide and Quercetin in solid lipid nanoparticles of Temozolomide and Quercetin for the treatment of Temozolomide associated cognitive impairment using RP-HPLC.
• Thiopurine therapy in inflammatory bowel disease: role of a spectrum of metabolites and enzymes in predicting the response using LC-MS/MS.
• Development of microneedle patch incorporated with insulin nanoparticles for the treatment of Type-I Diabetes Mellitus: Formulation development and in vivo monitoring.
• Implication Of Solution Phase Behavior On Bioavailability of Selected Anti-Tubercular And Anti-Retroviral Fixed Dose Combinations.
• Investigating The Influence Of Physicochemical Interactions On Pharmacokinetics Of Co-administered Anti-Malarial And Anti Retroviral Medications using LC-MS/MS.
• Exploring the utility of co-amorphous materials to concurrently improve the solubility and permeability of Fexofenadine.
• Investigating the influence of the type of polymer on sustaining the supersaturation from amorphous solid dispersions of Apremilast and its pharmacokinetics
• The significance of utilizing in vitro transfer model and media selection to study the dissolution performance of weak ionizable bases: an investigation using Saquinavir as a model drug.
• Investigation of drug-polymer miscibility, biorelevant dissolution, and bioavailability improvement of Dolutegravir-polyvinyl caprolactam-polyvinyl acetate-polyethylene glycol graft copolymer solid dispersions.

• A hands on experience on "Histopathological technique with various histopathological staining methods” held on August 11-13 2022 offered by MMMC organized by
  MAHE Student Research Forum, under the aegis of Directorate of Research, MAHE, Manipal.
• A hands on workshop on “Stem cell culture, functional characterization, and potency assays”. The workshop is scheduled on 20th February 2023 to 24th February 2023 organized by Manipal Centre for Biotherapeutics Research (MCBR), MAHE.
• A two week internship programme on bioanalytical method development and troubleshooting using LC-MS/MS ( Thermo TSQ Quantis Plus Triple Quadrupole, LTQ XL™ Linear Ion Trap Mass Spectrometers) at Ecron Acunova from July 31, 2023 to August 14, 2023 in the bioanalytical department.
  

1.PRAISE award, by Manipal Academy of Higher Education
February 2023. PRAISE (Publication and Research Award Incentive for Students to Excel) for the publications.
2. National Conference on Pharmaceutical Sciences , JSS college of pharmacy, Mysore April 2022. Awarded with best poster under the category of pharmaceutical quality assurance and pharmaceutics.
3. PGCET, by Andhra Pradesh June 2019 Got 82 Rank in PGCET entrance exam.

1. Dr. Krishnamurthy Bhat

Professor & Vice- Principal

Department of Pharmaceutical Quality Assurance, 

Manipal College of Pharmaceutical Sciences,

Manipal -576104, km.bhat@manipal.edu

+91 9845801575

2. Dr. Swapnil J Dengale

Assistant Professor 

Department of Pharmaceutical Analysis, 

National Institute of Pharmaceutical Education and Research (NIPER) 

Guwahati, Assam-781101 swapnil@niperguwahati.in

+91 9916311176

3. Dr. C. Mallikarjuna Rao

Professor & Principal

Department of Pharmacology

Manipal College of Pharmaceutical Sciences,

Manipal -576104,  mallik.rao@manipal.edu 

 +91-820-2922482

4. Dr. K Sreedhara Ranganath Pai

Professor

Department of Pharmacology

Manipal College of Pharmaceutical Sciences,

Manipal -576104,   ksr.pai@manipal.edu 

 +91-9886003726

I hereby solemnly declare that all the information provided here is to the best of my knowledge.

                                                                                                          Dani Lakshman Yarlagadda