Karthika Nair

• Preparing high-quality DNA libraries for Oxford Nanopore sequencing(MinIon & MK1C), using a range of library preparation kits, such as the Ligation Sequencing Kit, Rapid Sequencing Kit, or Native Barcoding Kit.
• Performing phenotypic and proteomic microbial identification using the Vitek 2 Compact and Vitek MS platforms, respectively, to accurately identify bacterial and fungal isolates from various environmental samples.
• Analyzing chromatograms and electropherograms generated by Sanger sequencing and interpreting the results based on relevant databases or annotation tools, such as NCBI.
• Conducting phylogenetic analysis of microbial data, utilizing a range of software and tools to perform comparative analysis of genetic sequences and construct evolutionary trees.
• Preparing comprehensive and accurate reports of Sanger sequencing and NGS results, including providing detailed information about the sample, experimental design, sequencing methodology, data quality, and interpretation of findings, and presenting the data in appropriate graphical or tabular formats.
• Interpreting microbial identification results based on relevant databases such as Vitek2 ID or Vitek MS MS/MS.
• Creating scientific posters for national conferences, utilizing a range of software and tools to produce clear and effective visual representations of research findings and writing abstracts for posters and presentations, adhering to conference guidelines and best practices for effective communication of scientific research.
• Coordinating and conducting skill development programs related to biotechnology and microbiology. Design and implement training modules for students and professionals, utilizing a range of teaching methods such as lectures, practical sessions, and online resources.

• Conducting microbiological experiments and analyzing data to meet project objectives and timelines of Project named “Technical Services in NCIM”
• Identification of microbial samples(bacteria, fungal, yeast, microalgae) received from Pharma companies as well as academics
• Performing various methods of genomic DNA isolation, including phenol-chloroform extraction, column-based purification, and bead-based extraction using magnetic beads or silica membranes.
• Optimizing PCR conditions to amplify specific genes of interest, such as 16S rRNA for bacteria or 18S rRNA for fungi and microalgae. Carrying out optimization of PCR annealing temperatures, extension times, and cycling conditions to maximize amplification efficiency.
• Analyzing PCR products using gel electrophoresis and spectrophotometry .
• Running capillary electrophoresis using automated sequencers, such as the Applied Biosystems 3500xl with different sequencing chemistries, such as BigDye and Brilliant Dye.
• Preparation of lyophilized vials of microorganisms and maintenance of glycerol stocks and performing quality control checks on the final product.

1. Acquiring laboratory techniques such as pipetting, centrifugation, gel electrophoresis, and microscopy.

2. Understanding and practicing techniques such as DNA and RNA isolation, PCR, such as isolation, purification, and characterization using methods like chromatography, electrophoresis, and spectroscopy .

3. Understanding how to use bioinformatics tools for sequence analysis, phylogenetic analysis, and other computational biology applications.

4. Learning to use and maintain various instruments such as spectrophotometers, microscopes, centrifuges, PCR machines , Applied Biosystems 3500xl Genetic Analyzer, Vitek2 Compact & Vitek MS.

5. Learning about quality control measures, such as Good Laboratory Practices (GLP), Good Manufacturing Practices (GMP), and Quality Assurance (QA) .