Summary
Overview
Work History
Education
Skills
Certification
References
Timeline
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Mohit Srivastava

Associate Scientist
Bengaluru

Summary

Biotechnology professional with over 8 years of experience in monoclonal antibody development, hybridoma technology, molecular biology, and mammalian cell culture. Proven expertise in developing and screening hybridoma clones, optimizing fusion protocols, and executing molecular biology workflows. Currently working at Syngene International in the Monoclonal Antibody Development Team, contributing to tool grade antibody development and learning recombinant MAb production in CHO cells.

Overview

13
13
years of professional experience
1
1
year of post-secondary education
3
3
Certifications
3
3
Languages

Work History

Associate Scientist

Syngene International
05.2021 - Current
  • Developed monoclonal antibodies using mouse hybridoma approach.
  • Performed immunogen QC, adjuvant mixing, and mice immunization.
  • Conducted retro-orbital bleeding, sera reactivity assessments, and PEG fusion.
  • Executed fusion screening, clone selection, limiting dilution, and small-scale batch culture.
  • Currently training in recombinant MAb development using CHO cells.
  • Enhanced overall efficiency within the lab setting through active participation in performance improvement initiatives.
  • Trained newly hired employees on processes and procedures relevant to lab tasks at hand.
  • Reagents inventory management.

Research Fellow

Mazumdar Shaw Cancer Center, Narayana Health City
07.2016 - 03.2020
  • Evaluation of Insulin Receptor Pathway as Target in Cancer Therapy.
  • qPCR - Insulin receptor expression profiling in cell lines, patient bone marrow samples.
  • Basic Molecular Biology.
  • Basic Mouse Hybridoma.
  • Reagents inventory management.

Junior Research Fellow

inStem, NCBS-TIFR
12.2014 - 08.2015
  • Studied calcium homeostasis in Mouse Embryonic Stem cells.
  • Conducted Mouse Embryonic Stem Cell culture and calcium staining assays.
  • Basic Molecular Biology - plasmid restriction digestion, plasmid DNA prep., transient transfection in HEK 293 cell line.
  • Trained interns and JRFs.
  • Reagents inventory management.


Trainee Scientist

Alkem Laboratories, R&D Centre
04.2014 - 06.2014
  • Developed cytotoxicity assay using A549 cell line

Junior Research Fellow

inStem, NCBS-TIFR
03.2012 - 04.2013
  • Mouse Genetics project involving generation of DNA constructs.
  • Molecular Biology - plasmid restriction digestion, plasmid DNA prep., ligation, transformation, basic cloning.
  • Mammalian cellculture - transient transfection in HEK 293 cell line.


Education

PG Diploma - Biotechnology

Karnataka State Open University
01.2013 - 01.2014

B.Tech - Biotechnology

SRM University

Skills

Protein Biology & Immunology:

Monoclonal antibody development (mouse hybridoma)- Mice immunization and sera reactivity testing, spleen harvest, PEG fusion, clone selection, Small-scale antibody production, Learning CHO cell-based recombinant expression

Molecular Biology: PCR, qPCR, RNA/cDNA prep, Plasmid DNA prep (mini/midi/maxi), Cloning, restriction digestion, ligation, transformation

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Certification

Qualified ICMR JRF (2015) – List A Candidate

References

Available upon request

Timeline

Associate Scientist

Syngene International
05.2021 - Current

Research Fellow

Mazumdar Shaw Cancer Center, Narayana Health City
07.2016 - 03.2020

Junior Research Fellow

inStem, NCBS-TIFR
12.2014 - 08.2015

Trainee Scientist

Alkem Laboratories, R&D Centre
04.2014 - 06.2014

PG Diploma - Biotechnology

Karnataka State Open University
01.2013 - 01.2014

Junior Research Fellow

inStem, NCBS-TIFR
03.2012 - 04.2013

B.Tech - Biotechnology

SRM University

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Mohit SrivastavaAssociate Scientist