Vinothini T
Bangalore
Summary
Experienced scientist with a strong background in research and analysis. Proficient in experimental design, data analysis, specializing in cell-based assays, molecular biology, assay development, and data analysis. Proven ability to design and execute experiments, optimize protocols, troubleshoot, and collaborate with cross-functional teams to advance product development. Skilled in using advanced laboratory techniques and data analytics to contribute to innovative solutions in drug discovery and bioanalytical research. My ability to work under tight timelines, coupled with an excellent work ethic, has enabled me to excel both individually and as part of a team. I seek opportunities to continue learning and growing within my field, applying my experience to enhance research and development efforts.
Overview
9
9
years of professional experience
Work History
Associate Scientist
Syngene International Limited
Bangalore
10.2024 - Current
- Assessed the efficacy of small molecules on various immune and cancer cells, and antiviral drugs on viability and cytotoxic response in a diverse panel of cancer cell lines.
- Developed experimental protocols and assays for scientific investigations, and conducted research using advanced laboratory techniques.
- Collaborated with cross-functional teams on project initiatives
- Analyzed data using specialized software tools and systems, and provided technical support and guidance to junior scientists.
- Analyzed data, interpreted results, and documented findings accurately in laboratory notebooks.
Senior Research Associate
Syngene International Limited
Bangalore
05.2022 - 10.2024
- Responsible for cell line maintenance and revival, and conducting various cell-based assays.
- Involved optimizing and performing immuno-oncology-based assays to determine the efficacy of novel antibodies and compound screening through high-throughput assays.
- Mentored junior researchers on laboratory techniques and best practices in experimentation.
- Managed inventory of laboratory supplies to ensure uninterrupted research operations.
- Documented and maintained detailed records of experimental protocols and data in Electronic laboratory notebooks.
Research Associate
TherogenBiologics Pvt. Ltd.
Chennai
07.2021 - 04.2022
- Focused on method development, optimization, validation, and report preparation for various analytical assays.
- My responsibilities included performing in-process samples and managing drug testing for R&D batches.
- Created and implemented work plans, and provided critical input on analysis and systems development.
- Trained junior staff members on proper laboratory techniques and procedures.
Research Associate
Theragen Molecular Innovation Pvt. Ltd.
Chennai
02.2021 - 06.2021
- Assisted in designing experimental approaches, developed methods required for testing molecules, prepared technical reports, and purified IgY.
- My role included the analysis of purified IgY and antibody fragments using the protein precipitation method, performing functional assays, preparing technical reports, detailed protocols, and handling QC documentation.
Junior Research Associate
Saksin Lifesciences Pvt. Ltd.
Chennai
04.2019 - 02.2021
- Engaged in assay development for novel biologics used for the optimization of process and yield from the upstream and downstream departments, affinity determination, and stability assays.
- Assisted in developing assays for in-process-related impurities and product-related impurities.
- Documented experimental results and maintained accurate records in databases.
- Maintained laboratory equipment and ensured compliance with safety protocols.
Research Executive
Saksin Lifesciences Pvt. Ltd.
Chennai
12.2016 - 03.2019
- Focused on biologics for drug discovery and development in treating wet AMD and other eye diseases by utilizing scrutiny and product development methodologies, enabling patients to access vaccines.
- Supported organization in maintaining work environment focused on scientific integrity and quality while fostering learning, open communication, collaboration and teamwork.
Education
M.Tech - Biopharmaceutical Technology
Anna University
Chennai01.2016
B.Tech - Biotechnology
Mepco Schlenk Engineering College
Tamilnadu01.2014
Skills
- Immuno-oncology functional assays: Binding and blockade assays, receptor density measurement, HiBit assay, ADCC, and CDC assays
- Mammalian cell culture: Cell line maintenance and revival, subculturing
- Primary cell line: Isolation of immune cells from murine tissues, including bone marrow, spleen, lymph nodes, and tumors; performed magnetic-based enrichment, activation, and expansion of T-cells, monocytes and macrophage polarization
- High-throughput cell-based assays: ELISA (96 and 384 well format), ALPHALISA, multiplex ELISA, cytotoxicity assays (MTT, CTG, and CytoTox Glo), proliferation assays
- Flow cytometry techniques: Multifactor flow cytometry assays (Novocyte, BD LSR Fortessa), extra and intracellular staining, panel designing, and cytokine profiling
- Live cell imaging: Incucyte (cell seeding optimization)
- Liquid handling and automation: Bravo, Echo 650 series liquid handler, multidrop-combi, and Integra Voyager
- Molecular biology techniques: Protein estimation (BCA, Bradford, and UV-spectrophotometer), gel electrophoresis (agarose, SDS-PAGE, and native PAGE), DNA/RNA manipulation techniques (DNA, RNA, and plasmid isolation, cDNA synthesis, bacterial transformation, cloning, restriction enzyme digestion, DNA ligation, RNA, and DNA quality assessment by Nanodrop, PCR, q-PCR, and RT-PCR), blotting (Western blot and Jess)
- Chromatography techniques: Reverse phase and SEC HPLC/UPLC
- Software tools and data analysis: GraphPad, SoftMax Pro, Excel, Gen5, OpenLab, and Lab Solutions for chromatography, and electronic lab notebook
Languages
Tamil, English
References
- Dr. Shiva Kumar Arumugasamy (shiva.cva16@outlook.com) +82-10-4824-2216, Assistant Professor (Research), Department of Chemical & Biochemical Engineering , Dongguk University, Seoul - 04620
- Renon, Arora, Lead Project Manager, arorareena12@gmail.com, +918840821929, Centre for cellular and Molecular Platforms, Bangalore, India
Projects
- Optimization of aqueous two-phase system for the purification of cellulase enzyme
- Cloning, expression, and immunoprophylactic evaluation of fused immunodominant regions of viral particle-2 of Infectious Bursal Disease Virus
Awards
Business achievement award-2024 Syngene International Ltd
Timeline
Associate Scientist
Syngene International Limited
10.2024 - Current
Senior Research Associate
Syngene International Limited
05.2022 - 10.2024
Research Associate
TherogenBiologics Pvt. Ltd.
07.2021 - 04.2022
Research Associate
Theragen Molecular Innovation Pvt. Ltd.
02.2021 - 06.2021
Junior Research Associate
Saksin Lifesciences Pvt. Ltd.
04.2019 - 02.2021
Research Executive
Saksin Lifesciences Pvt. Ltd.
12.2016 - 03.2019
M.Tech - Biopharmaceutical Technology
Anna University
B.Tech - Biotechnology
Mepco Schlenk Engineering College
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