Reshma Nair
Bengaluru
Summary
Professional researcher with passion for advancing scientific knowledge. Proven track record in designing and executing research experiments, contributing to published studies. Known for strong team collaboration and adaptability, with key skills in data collection and statistical analysis.
Overview
6
6
years of professional experience
1
1
Certification
Work History
Research Associate 2
Aurigene Oncology Ltd
07.2024 - Current
- Establish methods for rapid manufacturing of allogenic CAR-T for various lymphomas and leukemias.
- Optimize and standardize the established procedure for cost-efficiency and drive PD (process development) activities.
- Perform and validate QC tests – IFN gamma ELISA for testing potency against tumor, Vector Copy number of CAR gene by qPCR, flow cytometry to profile memory phenotype and CAR positivity and to understand the proportion of impurities in the final CAR-T product.
- Perform multicolor flow-cytometry and perform compensation and analyse data by using appropriate gating strategies, observing internal QA guidelines and regulatory guidelines and appropriate documentation.
- Perform multiplexing for several activating and inhibitory cytokines released during tumor and CAR-T cell co-culture and analyse using flow cytometry.
- Evaluate metabolic fitness of CAR-T cells using Seahorse from Agilent.
- A wholly owned subsidiary of Dr. Reddy's Laboratories
Research Associate II
CuraTeQ Biologics
04.2023 - 06.2024
- Execute and optimize bioassays- ELISA, reporter gene assays, flow cytometry based binding studies, for testing efficiency and Proof of Concept assays and biosimilarity analysis with reference products and RMPs, ADCC and ADCP assays.
- Develop and qualify methods for bioassays.
- Transfection of cells for cell line development for bioassays.
- Maintenance and instrument in-charge of CytoFLEX flow cytometer by Beckmann Coulter.
- Implementation of Good Documentation Practices.
- Aurobindo Biopharma Unit XVII
Senior Research Associate
Syngene International Ltd
10.2020 - 04.2023
- Execute and optimize bioassays for screening of bispecific and trispecific antibodies in various types of cancers and auto-immune disorders.
- Perform flow cytometry based effector profiling assays, binding efficiency, cell- viability assays, ADCC assays and analyse data using appropriate gating strategies.
- Execute cell-free assays such as ELISA and multiplexing; and cell-based- luminescence reporter assays.
- Execute and optimize IncuCyte (s3,sx5) -based kinetic ADCC assays using transfected cancer cell lines.
- Handle multiple adherent, semi-adherent and suspension cell lines, and their cryo- preservation.
- Engage in SQDECC and maintenance activities for NovoCyte flow cytometer as an additional responsibility and technical training of new joiners in the team.
Project Trainee
National Centre for Cell Science
11.2019 - 03.2020
- Worked on role of clathrin heavy chain in mouse embryonic stem cells and its cell cycle regulation.
- Performed transient transfection of mESCs cell line and western blotting.
- Engaged in team-building activities and project update discussions and presentations and actively participated in lab management activities.
Education
Master of Science - Biotechnology
Fergusson college
01.2020
Bachelor of Science - Biotechnology
KET’s Vinayak Ganesh Vaze College
01.2018
Skills
- Flow cytometry
- ADCC
- Binding efficiency
- Effector profiling
- Receptor density
- Immunostaining
- ELISA
- Cytokine release
- Cytokine inhibition
- IncuCyte-based assays
- Spheroid assays
- Luminex-based multiplexing
- Flow cytometry- based multiplexing
- Metabolic fitness assesment using Seahorse
- Luminescence reporter assay
- Antibody functional assays
- ADCP
- Reporter gene assays
- Mammalian cell transfection
- Monoclone selection
- Plasmid isolation
- Purification
- Microbial techniques
- Mammalian cell culture
- Adherent culture
- Semi-adherent and suspension culture
- Western blotting
- SDS PAGE
- Relative quantification
- AGE
- qPCR
Accomplishments
- Received Champion Award in Aurigene Oncology Limited for exhibiting exceptional pace in meeting deadlines and achieving milestones for the company.
- Received CEO Business achievement award in Syngene International, for successfully delivering significant data within a short timeline, enabling the client to file IPR within a shorter time-frame than was expected.
Certification
- Post Graduate Diploma Program in Data Science, GL Elevate from Great Learning
- Advance Excel, Internshala, 2019-07-01, BB80E641-E79E-3B57-06A2-2C5C27473D91, https://trainings.internshala.com/verify-certificate/
- Flow cytometry data analysis and presentation, FLOWCYTOMETRY SOLUTIONS Pvt. Ltd., 2022-08-01
Languages
English
Advanced (C1)
Hindi
Upper intermediate (B2)
Malayalam
Intermediate (B1)
Marathi
Intermediate (B1)
Timeline
Research Associate 2
Aurigene Oncology Ltd
07.2024 - Current
Research Associate II
CuraTeQ Biologics
04.2023 - 06.2024
Senior Research Associate
Syngene International Ltd
10.2020 - 04.2023
Project Trainee
National Centre for Cell Science
11.2019 - 03.2020
Bachelor of Science - Biotechnology
KET’s Vinayak Ganesh Vaze College
Master of Science - Biotechnology
Fergusson college
Instruments And Softwares Used
Flow cytometers- NovoCyte from Agilent, iQue3 IntelliCyt from Sartorius, CytoFLEX from Beckman Coulter, IncuCyte (s3, sx5) from Sartorius, Plate readers- Spark10M from Tecan, Synergy BioTek, SpectraMax, CLARIOstar, KingFisher FLEX for DNA isolation, GelDoc system from BioRad, ChemiDoc from BioRad, Spark from Tecan for DNA quantification, Seahorse from Agilent, Graphpad prism 8 and 9, FlowJo, NovoExpress, ImageJ, IncuCyte softwares, SoftMax Pro, CytExpert, CLARIOstar MARS
Personal Information
Title: Research Associate 2
Hobbies
Exploring stories in the form of movies or books, singing, reading, traveling