MUSKAN MANJARI

Designed and executed various experiments to investigate the role of TGF-B and TNF in NF-kB signalling pathway.

Looking into the role of microRNA in C15orf48.

Standardised various protocols for extraction and quantification of RNA ,DNA samples achieving high accuracy and reproducibility in molecular analysis of SDS-PAGE, qRT-PCR, Lentiviral Transduction, Reporter Assay, CRISPR-Cas 9

Practical expertise on Atomic Absorption Spectrophotometer (AAS)

Manganese pollution in eastern India causing cancer risk

• https://pubmed.ncbi.nlm.nih.gov/39562770/
• PMID: 39562770 PMCID: PMC11576912 DOI: 10.1038/s41598-024-78478-0

Microbial techniques: Handling of non-pathogenic cultures, different types of staining and streaking, antibiotic sensitivity, plasmid isolation. 

Cell culture: Handling of MCF7, 4T1, MDAMB231, HCT116, PANC-1, A549, Huh 7, HEK293T, NCI-H23, NCI-H650, THP 1 cell lines. 

Biochemistry: Different biochemical tests which differentiate gram positive and gram negative bacteria, TLC, analysis of proteins, paper chromatography, HPLC. 

Molecular/Biochemical techniques: Isolation of DNA, RNA, RT-qPCR, thermal cycler PCR, gel electrophoresis, western blotting, ChIP assay, recombination of DNA, immunoprecipitation. 

Immunological assays: ABO blood group determination, ELISA (sandwich and competitive). 

Instrumentation: Microscopy, different types of incubators, centrifuges, spectrophotometer, high performance liquid chromatography, RT-PCR, thermal cycler PCR, atomic absorption spectrophotometer (AAS). 

BIOINFORMATICS

Analyzing biological data, including DNA and protein sequences, using tools like NCBI BLAST, MEGA.

Society for Experimental Biology and Medicine, (Member as a student)

• 8th Bihar Science Conference, Poster presentation on fly ash - its impact on environment and its utilization in agronomic activities
• 21st Regional level National Children Science Congress, 2013, K.V. Samastipur (Bihar), Utilization of fly ash in construction purpose and in agriculture

• Cancer genomics
• Oncogenic signalling pathways responsible for the cause of onset of cancer
• How heavy metals are responsible in inducing breast cancer and colorectal caner.
• How genetically modified CAR T cell can be used as a targeted therapy to eradicate diseases.
• Benefits of CRISPR-Cas 9 in curing rare genetic diseases.

BIHAR BALSHREE of the year 2012 in BHARATNATYAM.

DIPLOMA in FINE ARTS

Blog: www.chemistrioflife@wordpress.