Nihar Bachan Das
Senior Scientist, Genome Informatics
Bengaluru
Summary
To secure a challenging position in Bioinformatics environment with committed and dedicated people to contribute my Bioinformatics and Computer Skills in the field of biological research to serve our nation. Organized and dependable candidate successful at managing priorities with a positive attitude.
Overview
8
8
years of professional experience
Work History
Scientist (Genome Infiormatics)
Genotypic Technology Pvt. Ltd
04.2022
- Working on Illumina and Nanopore based denovo and reference WGS analysis projects, GBS, Exome analysis, Genome Assembly, Functional Annotation, Variant calling (Germline, Somatic, Copy number, Structural) 5mC DNA Methylation, Insert Sequence Characterisation, AMR, VF Analysis, 16S Metagenome, Viral genome analysis etc
- Working on standardization of pipeline and setting analysis workflow for Nanopore Promethion sequence data.
Junior Scientist
Genotypic Technology Pvt. Ltd
03.2021 - 04.2022
- Worked on Illumina and Nanopore based denovo and reference WGS analysis projects, GBS projects, Exome analysis projects
- Genome assembly, Gene prediction, GO annotation, Pathway analysis, Variant calling, annotation etc
- Worked on various projects (WGS, Exome, MitoSeq, 16s Metagenome, SARS-CoV2 analysis)
- Analysed thousands of Covid samples and worked on pipeline standardization.
Senior Scientific Analyst
Genotypic Technology Pvt. Ltd
07.2020 - 03.2021
- Worked on Illumina and Nanopore based denovo and reference WGS analysis projects
- Worked on Exome projects (human: 15-20), (mouse: 2) and several WGS projects (other organism’s related).
Junior Scientific Analyst
Genotypic Technology Pvt. Ltd
01.2019 - 06.2020
- Worked on Illumina and Nanopore based denovo and reference WGS analysis projects, GBS projects, Exome analysis projects
- Genome assembly, Gene prediction, GO annotation, Pathway analysis, Variant calling, annotation etc.
Technical Officer
Ankur Seeds Private Limited
9 2015 - 12.2018
- Worked on denovo & reference based SNP calling, denovo assembly of reads, standalone batch blast, trait specific marker development using association analysis tool, genomic selection for hybrid development, allele specific primer designing, prediction of Synonymous/Non-synonymous SNPs & functional gene annotation.
Education
M. Sc. (Bioinformatics), 1st -
C.P.G.S, OUAT, Orissa University of Agriculture and Technology(OUAT)
Bhubaneswar, OdishaB.Sc. (Botany Hons), 1st - undefined
VikramDev Autonomous college, Berhampur University, Odisha
Jeypore, Odisha+2 Science, 3rd - undefined
DAV College, CHSE
Koraput, Odisha10th, 2nd - undefined
Modern English School, CBSE
Jeypore, OdishaSkills
Operating systems: Ubuntu-v22, Red Hat Linux9, MS-DOS, Windows95/98/2000/XP
Areas Of Interest
NEXT GENERATION SEQUENCING, GENOMICS (WGS, EXOME, GBS, DNA Methylation)
Bioinformatics Skills
- QC Analysis: FastQC, NanoPlot
- GBS: Tassel, Stacks
- Mapping Tools: Bowtie, BWA, Minimap2
- Variant Calling: BCF tools, Pilon+GATK, Varscan, CONTRA, Sniffles, Clair3, CuteSV, Pepper-Margin-Deepvariant
- Alignment: BLAST, MEGA, Clustalw
- Gene Prediction & Annotation: Augustus, Braker, Prokka, RAST
- GWAS Analysis: Tassel, Gapit(R env)
- Genome Assembly & Polishing: Masurca, Unicycler, Canu, Racon, Flye, SPADES, Pilon
- Assembly Validation: BUSCO, QUAST
- Genome Comparison: BRIG, Mauve, Symap
- BaseCalling, Alignment and DNA Methylation: Guppy-Minimap2-Modbam2bed
- AMR, VF, Transposon analysis: Abricate tool
- EPI2ME workflow: wf-human-variation (diploid variant calling, structural variant calling, aggregation of modified base counts, copy number variant calling, Short tandem repeat (STR) expansion genotyping), wf-metagenomics (16S metagenome analysis)
- HapDup: Diploid level assembly generation
- HapDiff: Structural variant caller for diploid level assembly
Personal Information
- Date of Birth: 07/16/91
- Gender: Male
- Nationality: Indian
- Marital Status: Unmarried
Domain Expertise
Have expertise in handling standard and R&D projects ((Insert Sequence Characterization, CRISPR editing, Small Tandem Repeat based genotyping etc) associated with Whole genome sequencing data for both short (Illumina) and long reads (Nanopore) platform. Execution of publicly available Nextflow and Docker related workflows for WGS data analysis.
Extracurricular Activities
Playing Cricket, Net surfing, Listening Music
Projects
Carried out M.Sc dissertation work on P.infestans genome data analysis & gene prediction employing various bioinformatics (NGS)tools under guidance of Dr. Soma S Marla (Principal Scientist) at Bioinformatics Laboratory, Division of Genomic Resources, National Bureau of Plant Genetic Resources, ICAR, Pusa campus, New Delhi
Disclaimer
I hereby declare that the above mentioned information is correct up to my knowledge and I bear the responsibility for the correctness of the above mentioned particulars.
Timeline
Scientist (Genome Infiormatics)
Genotypic Technology Pvt. Ltd
04.2022
Junior Scientist
Genotypic Technology Pvt. Ltd
03.2021 - 04.2022
Senior Scientific Analyst
Genotypic Technology Pvt. Ltd
07.2020 - 03.2021
Junior Scientific Analyst
Genotypic Technology Pvt. Ltd
01.2019 - 06.2020
Technical Officer
Ankur Seeds Private Limited
9 2015 - 12.2018
M. Sc. (Bioinformatics), 1st -
C.P.G.S, OUAT, Orissa University of Agriculture and Technology(OUAT)
B.Sc. (Botany Hons), 1st - undefined
VikramDev Autonomous college, Berhampur University, Odisha
+2 Science, 3rd - undefined
DAV College, CHSE
10th, 2nd - undefined
Modern English School, CBSE
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