SPSS
Pallavi Mudgal
Meerut
Summary
To seek and maintain full-time position that offers professional challenges utilizing interpersonal skills, excellent time management and problem-solving skills. Organized and dependable candidate successful at managing multiple priorities with a positive attitude. Willingness to take on added responsibilities to meet team goals.
Overview
1
1
year of professional experience
11
11
years of post-secondary education
2
2
Languages
Work History
Trainee
Lal Bahadur Shastri Building, IARI, PUSA Campus
2018.06 - 2018.07
- Completed research, compiled data, updated spreadsheets, and produced timely reports.
- Obtained informed consent of research subjects or guardians.
- Collaborated with research teams to develop project plans aligned with target timescales.
- Allocated and managed laboratory space and resources.
- Collaborated with researchers and scientists to design and execute experiments.
- Participated in lab meetings and project presentations.
- Monitored and documented safety standards to verify compliance.
- Prepared reagents and solutions following standard laboratory formulas and procedures.
- Adhered to laboratory safety procedures to maintain compliance with quality control standards.
- Prepared samples for analysis by performing complex sample preparation tasks.
- Provided support and guidance to junior lab staff.
- Operated and performed preventative maintenance on laboratory equipment and instruments.
Trainee
Department Of Plant Molecular Biology, Delhi University, South Campus
2017.05 - 2017.06
- Maintained overall safe work environment with employee training programs and enforcement of safety procedures.
- Analyzed problems and worked with teams to develop solutions.
- Completed research, compiled data, updated spreadsheets, and produced timely reports.
- Attended training courses to build understanding of processes, techniques, and industry.
- Learned new materials, processes, and programs quickly.
Education
Ph.D. - Biotechnology
Banasthali Vidyapith
Rajasthan, India 2020.08 - 2024.05
Diploma in Computational Biology -
Banasthali Vidyapith
Rajasthan, India 2018.07 - 2019.05
Master of Science -
Banasthali Vidyapith
Rajasthan, India 2017.07 - 2019.05
Bachelor of Science -
Banasthali Vidyapith
Rajasthan, India 2014.07 - 2017.05
Intermediate/+2 -
S.D. Public
Uttar Pradesh, India 2013.07 - 2014.01
Matriculation/10th -
S.D. Public
Uttar Pradesh, India 2011.07 - 2012.04
Skills
Project Support
Accomplishments
- 3rd International Convention on Green Environment, Technology & Entrepreneurship through Innovation ICGETEI 2023 (Hybrid Format)
- Poster presentation at the International Conference on "Biotechnological Interventions to Overcome the Challenges of Covid/ Post-Covid Era (BIOCoPE2022)" (Online)
- One-month International Workshop on Next-Generation Sequencing Analysis
- TEQIP-III organized a short-term "Spectroscopic Techniques for Material Characterization" course at MNIT, Jaipur
- Best oral presentation at National Conference of Computational Biology, Banasthali Vidyapith
- Master in Biotechnology Sponsored under the fellowship of the Department of Biotechnology (DBT)
Masters Research Summary
1. Phytochemical screening of various parts of Chenopodium murale using different Soxhlet extracts was conducted to identify the most effective extract with antimicrobial properties.
2. The biological synthesis of ZnO and Fe2O3 nanoparticles using Chenopodium murale ethanol leaf extract was reported as a sustainable alternative to traditional chemical methods.
3. The synthesized nanoparticles were characterized using XRD, UV-vis, SEM, TEM, and FT-IR spectroscopy, demonstrating their stability and confirming their composition.
4. Examination of the morphology revealed the nanoparticles' spherical nature, while EDXS analysis indicated their high purity.
5. Antibacterial and antifungal tests using the agar diffusion method exhibited notable antimicrobial activities of ZnO and Fe2O3 nanoparticles against various microorganisms, including Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, Aspergillus niger, and Penicillium chrysogenum.
6. The study highlighted the eco-friendly and straightforward green synthesis approach for producing multifunctional ZnO and Fe2O3 nanoparticles, emphasizing their potential as antioxidant agents through DPPH, FRAP, and Metal Chelating activity tests.
Technical Abilities
- Animal handling: Rats, Mice, Guinea Pigs, Rabbits
- Molecular analysis: Isolation and estimation of genomic DNA/RNA, cDNA synthesis, PCR amplification, carried out agarose and polyacrylamide gel electrophoresis, quantitative real-time PCR of expression analysis of heart, liver, and hippocampus-specific genes marker.
- Toxicity studies: Blood glucose measurements, serum ALP, cholesterol, creatinine, serum glutamic oxaloacetate transaminase (SGOT), serum glutamic pyruvate transaminase (SGPT) and triacylglycerides. Histology of pancreas, uterus, ovary and microtomy of femur and tibia bones. Estimation of biochemical markers of SOD, GSH, TBARs, NO, Protein carbonyl and Catalase.
- Histochemistry: Localization of pancreatic islets of Langerhans), hepatocellular cells, kidney glomeruli, cardiac fibers, Pneumocytes
- Proteomics: Isolation and estimation of proteins, PAGE-SDS & Native.
- Nanotechnology: Expertise in both chemical and biosynthesis methods for nanoparticle synthesis, along with their characterization and conjugation with various biomolecules, Proficient in a range of analytical techniques, including HPLC, TLC, HPTLC, UV-Spectroscopy, XRD, SEM, and TEM.
- Tissue Cutlture Techniques: Skilled in tissue culture, with experience in maintaining HeG2, A549 lung adenocarcinoma, and U87 MG glioblastoma cell lines. Competent in sub-culturing, performing Cell Viability Assays (MTT), and staining techniques such as Trypan Blue and MitoTracker Green
- Applied Microbiology: Applied expertise in various microbiology techniques, including bacterial isolation, determination of Minimum Inhibitory Concentration (MIC), bacterial and fungal cultures, media preparation, as well as streaking and spreading techniques
- Softwares: Graphpad prism version 8.02, Mendley cite manager, Biorender, Chemdraw, Autodocktool.
Subject Of Academics Interest
- Research Methodology
- Research ethics and Publications
- Cell and Molecular Biology
- Concepts of Genetics
- Applied Microbiology
- Immunology
- Analytical techniques of Biotechnology
- Biochemistry
- Bioinformatics and Computational Biology
- Plant Biotechnology
- Animal Biotechnology
- Recombinant DNA technology
- Environmental Biotechnology
Areas Of Research Interest
- Plant Biotechnology
- Plant Molecular Biology
- Microbiology
- Recombinant-DNA technology
- Nano-Biotechnology
- Regenerative medicine
- Stem cells
- Animal biotechnology
Phd Research Summary
Throughout my Ph.D., I explored the therapeutic applications of isolated mitochondria in various metabolic disorders, including diabetes, non-alcoholic fatty liver disease, CCl4-induced liver injury, and Alzheimer's disease, utilizing Wistar rats and Swiss albino mice as animal models. In my research, I independently conducted a comprehensive array of laboratory experiments, primarily in biochemistry, evaluating oxidative stress parameters such as SOD (superoxide dismutase), CAT (catalase), GSH (glutathione reductase), MDA (malondialdehyde-lipid peroxidation), NO (nitric oxide), and Total Protein, along with assessing mitochondrial enzyme complex activities. Additionally, I extensively employed essential molecular biology techniques such as RT-PCR, RNA isolation from animal tissues, cDNA preparation, mitochondria isolation, mtDNA isolation, and mtDNA copy number determination, often using gel electrophoresis for analysis. Furthermore, I delved into evaluating the impact of mitochondria transplantation on vital physiological parameters, including blood glucose levels, insulin tolerance, oral glucose tolerance, body weight, BMI, waist circumference, blood pressure, total cholesterol, and lipid profiling. My research also included investigating inflammatory markers like IL-6 and NFkB and exploring bioenergetics and mitochondria biogenesis units, specifically ATP and PGC1 alpha. During my studies, I synthesized nanoparticles such as Graphene quantum dots and superparamagnetic iron oxide, applying chemical techniques to track intravenously transplanted mitochondria in diabetic rats and analyze their biodistribution within the tissues. I gained proficiency in various characterization techniques to track intravenously transplanted mitochondria in diabetic rats and analyze them, using SEM (scanning electron microscopy), TEM (transmission electron microscopy), UV-Vis spectroscopy, FT-IR spectroscopy, photoluminescence spectroscopy, and X-ray diffraction for thorough analysis. Moreover, my work involved the study of diverse cell lines such as HepG2 (hepatic cells), U87MG (neuronal cells), A549 (sarcoma cells), and THP1 (human leukemia monocytic cells). I developed expertise in their passaging and subculturing while observing and analyzing morphological differences using an inverted microscope. Additionally, I performed staining procedures with trypan blue and mitotracker green, conducted cell counting, and utilized cytotoxicity assays (MTT) to evaluate the effects of different nanoparticles. I was responsible for performing data and statistical analysis and participated in writing papers where results were published. I also actively collaborated with my research supervisor to direct future experiments to find the answers to our research questions, conception, design, and execution to address defined problems and also to maintain clean, organized, and well-stocked laboratory environments. I presented my research findings to scientific and general audiences and published my work in peer-reviewed journals.
Extracurricular Activities
Street plays 2nd position in inter-school Volleyball tournament Semi-finalist at district-level Volleyball Championship Novel reading (Sci-fi and exploratory, history) Interested in politics and history
Training
- Department of Plant Molecular Biology, Delhi University, South Campus, New Delhi
- Department of Biotechnology, Sardar Vallabh Bhai Patel, Meerut
- Lal Bahadur Shastri Building, IARI, PUSA campus, New Delhi
- Hands-on session in Computational Biology, Banasthali Vidyapith, Rajasthan
Software
Graph Pad 8.02
Mendley
Biorender
Chemdraw professional
Interests
Sports
Dramatics
Timeline
Ph.D. - Biotechnology
Banasthali Vidyapith
2020.08 - 2024.05
Diploma in Computational Biology -
Banasthali Vidyapith
2018.07 - 2019.05
Trainee
Lal Bahadur Shastri Building, IARI, PUSA Campus
2018.06 - 2018.07
Master of Science -
Banasthali Vidyapith
2017.07 - 2019.05
Trainee
Department Of Plant Molecular Biology, Delhi University, South Campus
2017.05 - 2017.06
Bachelor of Science -
Banasthali Vidyapith
2014.07 - 2017.05
Intermediate/+2 -
S.D. Public
2013.07 - 2014.01
Matriculation/10th -
S.D. Public
2011.07 - 2012.04
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