M Parusharamulu

Reaction phenotyping (Chemical inhibition method, rCYPs, rUGTs, rFMO and rMAO)

Knowledge on RAF and ISEF

Experience in conducting client projects

Preparation of liver and intestinal S9 and microsomes

505 (b) (2) concepts

Preparation of SOPs

Data monitoring and interpretation

Data communication to the cross functional teams

Invitro release test (IVRT)_Franz diffusion cells (Permeability assay_porcine buccal and sublingual)

Validation and development of UGTs and FMO

HPLC-2030C; UV and PDA, Lab solution software

Preparation of SOW, protocols and costing of the assay (for service projects)

Preparation of reports

pH dependent solubility

Handling of 16 channel pipette

Handling of Integra voyager pipette.

Hepatocytes metabolic stability

Validation and development of MAO inhibition

Determination of reversibile and irreversible inhibition

KI, Kinact determination

Mechanism based inhibition

ERP and SAP system (Indenting Chemicals and biologicals for ADME studies)

Enzyme kinetics

PAMPA

Microsomal metabolic stability(Hepatic and extra-hepatic tissues)

Intrinsic clearance (CLint)

Blood and plasma stability

Brain stability

Determination of unbound fraction

Blood to plasma portioning ratio.

Protein estimation

Invitro to invivo hepatic clearance prediction (IVIVE)

Bioanalytical method development and validation-HPLC Shimadzu (Class-VP)

Handling of Single channel and multichannel transferpette

Routine calibration of HPLC, weighing balance, pH meter and single channel transferpette

Liquid extraction

Metabolic stability

CYP inhibition

Aqueous Solubility

LogD

Blood collection from rats and preparation rat brain homogenation