Raghavi R
Scientist I
Bangalore
Summary
Results-driven biotechnology professional with a solid foundation in R&D, specializing in molecular biology and recombinant protein technology. Expertise includes cloning, transformation (E. coli, Pichia pastoris), and advanced protein expression techniques, such as inclusion body handling, refolding, and purification through various chromatography methods (ÄKTA/NGC FPLC). Proficient in protein characterization techniques, including SDS-PAGE, SEC-HPLC, ELISA, and Western blot, as well as analytical methods like UV-Vis spectrophotometry and fluorometric assays. Demonstrated skills in assay development, protocol optimization, troubleshooting, and GLP-compliant documentation, complemented by strong collaboration and mentoring abilities.
Overview
2
2
years of professional experience
4
4
Languages
Work History
Scientist Level I
BioArtha Labs Pvt Ltd
08.2024 - Current
- Optimized recombinant protein expression in E. coli and Pichia pastoris, improving protein yield, solubility, and overall expression efficiency.
- Purified recombinant proteins using affinity (Ni-NTA), ion-exchange, and size exclusion chromatography (SEC) via AKTA/NGC systems, achieving high purity and consistency for downstream applications.
- Performed protein characterization using SDS-PAGE and SEC-HPLC, ensuring batch-to-batch consistency, structural integrity, and quality standards.
- Validated protein functionality through ELISA and Western blot, supporting reliable assay performance and downstream analytical applications.
- Designed and executed molecular biology workflows including cloning, transformation, plasmid construction, PCR, and RT-PCR, ensuring accurate construct validation and reproducible results.
- Contributed to assay development and reverse engineering of diagnostic systems, improving assay performance and adaptability to project-specific requirements.
- Enhanced experimental reproducibility and workflow efficiency by troubleshooting and optimizing protocols across molecular and protein workflows.
- Maintained accurate, GLP-compliant documentation (experimental data, QA records, SOPs), ensuring regulatory compliance and traceability.
- Collaborated effectively with cross-functional teams to deliver project milestones within timelines, supporting seamless project execution.
- Trained and mentored new team members, improving team efficiency and adherence to laboratory and compliance standards.
Technical Experience
BioArtha Labs Pvt Ltd
05.2024 - 03.2026
- Optimized PCR reaction conditions and validated Phusion-based mastermix formulations, improving amplification efficiency, specificity, and overall assay performance.
- Developed and standardized BCA protein quantification assays (macro and micro formats), enhancing sensitivity, reproducibility, and consistency across experiments.
- Performed quality control and functional validation of recombinant molecular biology enzymes using activity-based and PCR-based assays, ensuring reliable enzyme performance.
- Conducted fluorescence-based enzymatic assays, including exonuclease activity using molecular beacon systems, enabling accurate enzyme characterization and validation.
- Performed dsDNA quantification using Hoechst dye-based fluorescence assays, ensuring sensitive and accurate nucleic acid measurement.
- Optimized buffer formulations and resolved stability issues, improving reagent reliability and assay consistency.
Intern – Molecular Biology
BioArtha Labs Pvt Ltd
05.2024 - 07.2024
- Assisted in recombinant protein expression and purification workflows.
- Performed SDS-PAGE, Western blotting, and ELISA for protein analysis.
- Supported research documentation and basic data analysis.
Education
Master of Science - Biotechnology
Lady Doak College
Madurai06-2024
Bachelor of Science - Biotechnology
Lady Doak College
Madurai06-2022
Skills
Molecular Biology: PCR, RT-PCR, DNA/RNA Isolation, Plasmid Isolation, Cloning & Transformation (E coli, Pichia pastoris, Bacillus licheniformis)
Protein Expression & Purification: Recombinant Protein Expression, Inclusion Body Handling & Refolding, Affinity(Ni-NTA), Ion-Exchange & Size Exclusion Chromatography (SEC), FPLC (ÄKTA, Bio-Rad NGC)
Protein Characterization: SDS-PAGE, SEC-HPLC, ELISA, Western Blot, Protein Assays
Filtration & Processing: Tangential Flow Filtration (TFF), Hollow Fiber Filtration (HFF), Membrane Filtration
Instrumentation and Analytical Techniques: High-Pressure Homogenizer, Probe Sonicator, Lyophilizer, UV-Vis Spectrophotometry, Nanodrop, Fluorometer
Software & Tools: Microsoft Office, PyMOL, Basic Bioinformatics Tools
Professional Skills: Experimental Design, Troubleshooting, QA Documentation (GLP), Scientific Writing, Team Collaboration, Time Management, Leadership
Timeline
Scientist Level I
BioArtha Labs Pvt Ltd
08.2024 - Current
Technical Experience
BioArtha Labs Pvt Ltd
05.2024 - 03.2026
Intern – Molecular Biology
BioArtha Labs Pvt Ltd
05.2024 - 07.2024
Master of Science - Biotechnology
Lady Doak College
Bachelor of Science - Biotechnology
Lady Doak College
Involvement
- President, SIBS Club, Department of Biotechnology, Lady Doak College, Madurai
- 2023-24
- Vice President of the Student Cabinet, Student Cabinet, Lady Doak College, Madurai
- 2021-22
- Library Volunteer, Department of Biotechnology, Lady Doak College, Madurai
- 2020-21