Rahul Thakur

• Planning and execution of developmental batches at AMBR and 5L Bioreactor scale to improve glycan profile and charge variants profile of monoclonal antibodies
• Achieved project objectives of tuning a mAb Glycan profile for various biosimilar molecules by DoE-based process development for a better understanding of the effect of critical process parameters on cell growth, antibody production, and glycan distribution.
• Author technical documents, including protocols, reports, SOPs and risk assessments, Data Analysis and Data Interpretation

• Performed small-scale bioprocess experiments for upstream (cell culture) operations, including cultures in shake flasks and benchtop bioreactors to improve product titer and quality profile of mAbs.
• Conducted Multivariate Analysis of Cell Culture Bioprocess Data for process scale-up, real-time process monitoring, and process control.
• Conducted mammalian cell culture batches at the pilot plant scale of 20L SS bioreactors scale to establish Process consistency.

• Performed experiments for productivity and product quality optimization in shake flask and bench-top bioreactor (improved protein titer by 57%)
• Developed in-house feed formulations and evaluated against commercial feeds in Fed-Batch Culture Process.
• Improved feed formulation and cell culture process efficiency by altering the process temperature.

Project Title: Potential transdifferentiation between hepatocytes and cholangiocytes during liver tumorigenesis in transgenic zebrafish.

• Investigated and analyzed the transdifferentiation event between hepatocytes and cholangiocytes during liver tumorigenesis in zebrafish (treated with doxycycline and 4-OHT)
• Discovered the transdifferentiation of cholangiocytes to hepatocytes during tumorigenesis