Rohit Kumar Khetan
Research Fellow
Raigarh
Summary
- Accomplished research fellow with extensive experience in molecular biology, cell culture, and quantitative research. Demonstrated expertise in developing and validating bioassays, handling various cell lines, and conducting high-level research on diseases such as COVID-19, Avian Influenza Virus, and Bovine Viral Diarrhea Virus. Proven track record in setting up molecular hematopathology labs, testing cancer patient samples, and mentoring junior scientists. Adept at using RT-PCR, qRT-PCR, fragment analysis, Sanger sequencing and NGS techniques. Committed to advancing scientific knowledge through innovative research methodologies and effective project management. Career goal: to contribute to groundbreaking research in biomedical sciences while fostering the development of emerging scientists.
Overview
11
11
years of professional experience
5
5
years of post-secondary education
2
2
Languages
Work History
Research Fellow
Bar Ilan University
06.2023 - 12.2024
- Working on the effect of exosome-secreted proteins on the social motility in Trypanosoma brucei
Scientific Officer-C
Homi Bhabha Cancer Hospital
01.2022 - 04.2023
- Lab setup for molecular hematopathology and started the testing of cancer patients samples against Acute Myeloid Leukemia (AML) and Chronic Myeloid Leukemia (CML)
- Skills: RT-PCR, qRT-PCR, Fragment Analysis and Sanger Sequencing
Executive-Cell Culture
Genesy'S Biologics Pvt. Ltd.
05.2021 - 09.2021
- Bioassays method development and validation
- Expertise in cell-based assay of Glucose uptake, Mitogenic and Autophosphorylation assays
- Skills: Handling and maintaining 3T3L1, HepG2, SaoS2 and CHO-K cell lines
Project Associate- II
CSIR- IITR
06.2020 - 04.2021
- Testing for Corona Virus Diseases (COVID-19)
- Skill: BSL +3 experience, qRT-PCR
Research Fellow
Bar Ilan University
10.2018 - 09.2019
- Study of different factors influencing exosome secretion in Trypanosomatids
Young Professional
ICAR-NIHSAD
03.2018 - 09.2018
- Testing for Avian Influenza Virus like hemagglutination assay (HA) and hemagglutination inhibition (HI)
- Skill: BSL+3 experience, qRT-PCR
Research Assistant
All India Institute of Medical Sciences
11.2016 - 08.2017
- Genotypic and phenotypic alterations in primary open angle glaucoma (POAG)
Junior Research Fellow
ICAR-National Institute of High Security Animal Diseases
01.2015 - 09.2016
- Master Thesis: Prevalence and Molecular Epidemiology of Bovine Viral Diarrhea (BVD) in Ruminants with Special reference to Mithun (Bos frontalis) in North Eastern States of India
Trainee
B.R. Ambedkar Center for Biomedical Research
01.2014 - 07.2014
- Expression of Aldosterone Synthase Protein (CYP11B2) in Patients with Essential Hypertension: A Pilot Study
Education
M.Sc. - Biotechnology
Jaipur National University
Jaipur 08.2012 - 05.2014
B.Sc. - Biotechnology
Devi Ahilya University
Indore 07.2008 - 05.2011
Skills
Molecular Biology Technique : SDS PAGE, qRT-PCR, PCR, Western and Northern Blotting, Flow cytometry, DNA sequencing, Fragment analysis, NGS, Cloning
Cell Culture: Maintain different mammalian and parasite cell lines
Microscopy: Scanning electron microscope, Time lapse microscope, STED microscope and confocal microscope
Honorsandawards
- Milgat Hanasi Fellowship (President Fellowship), 06/01/23
- Travel grant for Germany by European Cooperation of Science and Technology, 07/01/24
- Certificate of appreciation- Contribute in COVID-19 testing facility, 11/01/20
Publications
- Identification of bovine viral diarrhoea virus type 2 (BVDV-2) in cattle bull semen from Southern India and its genetic characterization., CURRENT SCIENCE, VOL. 114, NO. 3, 02/10/18
- Serological evidence of bovine viral diarrhea virus (BVDV) infection in farmed and free ranging mithun (Bos frontalis) in the North Eastern hilly region of India., Trop Anim Health Prod., 08/01/17
- Serological evidence of widespread West Nile Virus and Japanese Encephalitis Virus infection in native domestic ducks (Anas platyrhynchos var domesticus) in Kuttanad region, Kerala, India., Comp Immuol Microbiol Infect Dis., 10/01/16
- Highly Pathogenic avian influenza H5N1 virus induces cytokine dysregulation with suppressed maturation of chicken monocyte-derived dendritic cells., Microbiol Immunol., 10/01/16
Training
- SBS- ASM- ICAR Biosafety Awareness Programme, including Workshops on 'Culture of Responsibility, Pathogen Inventory Management and Fundamentals of Working with Biosafety Cabinets.
- 9th Symposium on 'Frontiers in Biomedical Research.
- International Year of Chemistry-2011, 'Expanding Horizons in Science', International Symposium on Research in Basic Sciences.
Timeline
Research Fellow
Bar Ilan University
06.2023 - 12.2024
Scientific Officer-C
Homi Bhabha Cancer Hospital
01.2022 - 04.2023
Executive-Cell Culture
Genesy'S Biologics Pvt. Ltd.
05.2021 - 09.2021
Project Associate- II
CSIR- IITR
06.2020 - 04.2021
Research Fellow
Bar Ilan University
10.2018 - 09.2019
Young Professional
ICAR-NIHSAD
03.2018 - 09.2018
Research Assistant
All India Institute of Medical Sciences
11.2016 - 08.2017
Junior Research Fellow
ICAR-National Institute of High Security Animal Diseases
01.2015 - 09.2016
Trainee
B.R. Ambedkar Center for Biomedical Research
01.2014 - 07.2014
M.Sc. - Biotechnology
Jaipur National University
08.2012 - 05.2014
B.Sc. - Biotechnology
Devi Ahilya University
07.2008 - 05.2011