Years of post PhD
S KRISHNAN
R&D Molecular Biology
HYDERABAD
Summary
Effective advisor communicates well with people of all ages and backgrounds. Excellent eye for detail enabling quick identification of areas for improvement and suggesting strategies to help businesses reach goals. Focused on introducing efficient systems and processes.
Overview
12
12
4
4
Certifications
Work History
Head R&D / Consultant Scientist
BioArtis Life Sciences/Sentier Consulting & Healthcare
HYDERABAD, TELANGANA
2023.07 - Current
- As Head Molecular Biologist and R&D, I lead a team of six researchers from different fields of molecular biology/ biochemistry/ pathology
- Design Cloning, RT-PCR, Expression vector design, in-vitro RNA synthesis
- Protein expression and purification and check the activity of the expressed enzymes, and development of various indigenous kits
- Working on development of in-house Secondary antibodies conjugated with Horse Radish Peroxidase
- Using Snap gene software to design constructs
- We're making DNA, RNA, and protein markers/ladders of various molecular weights
- We’re in the midst of developing in-house versions of various restriction enzymes used in Molecular Biology and Biotechnology
- Develop CHO, HEK, cell lines for protein production
- Perform cell line genetic characterization using methods such as real-time PCR, NGS, and Southern blot to support cell line development and clone selection
- Manage development projects with outsourcing activities.
- Assisted with day-to-day operations, working efficiently and productively with all team members.
- Skilled at working independently and collaboratively in a team environment.
- Adaptable and proficient in learning new concepts quickly and efficiently.
- Provided professional services and support in a dynamic work environment.
- Self-motivated, with a strong sense of personal responsibility.
Research Scientist
Bioartis life sciences private Ltd
HYDERABAD, TELANGANA
02.2019 - 12.2019
- Project Design: Improvement of various products supplied by Bio Artis life sciences private Ltd
- Assist in the development of new products and technologies in support of Loop-mediated Isothermal Amplification (LAMP) PCR kits and implementing these kits as point-of-care diagnostic tools for rapid and sensitive detection pathogens such as White spot syndrome virus (WSSV), Enterocytozoon hepatopenaei (EHP) in shrimps and Human papillomavirus (HPV), & Tuberculosis (TB) in humans
- Perform and troubleshoot qPCR/real time PCR assays that support process development
- Documentation: Followed regulatory requirements to develop daily reports and document findings into laboratory information systems
- Analysed data gathered from junior research associates.
- Utilized expertise in microbiology, chemistry, and hematology to help with diagnosis of aqua culture related issues issues.
- Learned new laboratory techniques and applied expertise in carrying out enhanced experiments under supervision of senior lab members.
- Followed health and safety regulations while handling dangerous or toxic substances.
Head – Molecular Biologist
Genes2me
Gurugram, Haryana
02.2022 - 06.2023
- As Head Molecular Biologist and R&D, I lead a team of eight researchers in various fields of molecular biology
- I along with the team was involved in developing in-house versions of various restriction enzymes/ other enzymes used in Molecular Biology and Biotechnology
- Molecular cloning has advanced from the cloning of a single DNA fragment to the assembly of several DNA components into a single contiguous stretch of DNA
- The goal of new and developing technology is to make cloning as simple as stacking 'blocks' of DNA adjacent to each other
- Methods for assembling DNA: Multiple DNA fragments can be assembled in a single tube reaction using a variety of modern, elegant technologies
- Furthermore, the capacity to combine several DNA fragments in a single tube streamlines and streamlines a previously inefficient set of restriction/ligation procedures
- We are now working on Gibson synthesis or assembly protocol
- We're working on our own Gibson assembly kit, using enzyme components that we make in-house
- Have used Infusion cloning technique to clone multiple fragments (up to 10 fragments) at a time.
- Scheduled and trained technical staff members.
- Conducted experiments related to cell culture.
- Ordered laboratory equipment and supplies.
- Managed overall laboratory functions.
Solutions leader (Subject matter expert (Molecular biology))
Brane Enterprises private limited
HYDERABAD, TELANGANA
12.2019 - 12.2021
- The company is involved in 'Natural Solution Language' dependant solutions development based on no code platform
- Involved in a team related to development of modules related to CDSS using different databases
- To arrive at a computational framework for the creation of maps of molecular connectivity in any given disease context
- The system consists of three components: network creation, recovery of text and extraction of information, and mapping of molecular connectivity
- Mapping Concepts in biology in terms of Conceptual frame work designed and desired by the company platform, that is being developed based on 'Natural solutions Language'.
- Worked alongside eam leaders to recognize and address technical dependencies causing performance bottlenecks.
- Simplified complex technical solution frameworks into digestible documentation and presentations for consumption by senior leadership.
- Developed and designed software for variety of applications, supported by a team of soft ware professionals
- Analyzed user requirements and developed solutions to meet needs.
Molecular Biology Research and Development
GCC Biotech
Kolkata , West Bengal
09.2018 - 02.2019
- Was head of the Molecular biology lab consisting a team of five research fellows
- Worked with management to evaluate future projects and align with critical path schedule requirements.
- Optimized existing formulation and processes to improve product performance, process robustness and safety while reducing costs.
- Seek, develop and qualify new technologies, reagents and assays
- Leader of the Molecular Biology team that will plan, manage and execute and execute pre-clinical development activities related to Molecular Biology programs
- Project Design: Improvement of various products supplied by GCC biotech, especially involved in making new versions of TAQ polymerase using Site directed mutagenesis
- Identified and evaluated potential life science investment opportunities.
- Quality Evaluation: Designing experiments towards development and validation of various kits (supplied by GCC Biotech Ltd such as RNA isolation kits, DNA isolation kits) using standard biochemical, cellular & molecular biologic techniques
- Key Research: Making of ladders (100bp, 1 Kb etc), preparing fusion proteins (Phusion TAQ), Revival, Passaging, Maintenance and Cryopreservation of cell (Hela, HEK, Jurkat, K562 etc.), DNA fragmentation by ladder formation assay, developing kits based on Ligase free cloning
- In the process of developing indigenous kit based on COBAS TaqMan HCV test for the quantitation of hepatitis C viral (HCV) RNA, HIV & HBV in human plasma for one of their BioPharma clients
- Polymerase Chain Reaction: Performed and interpreted multiple real-time PCR assays in order to detect and characterize potential pathogens and ensure assay quality
- Maintained chain of custody and sample integrity from DNA extraction through qPCR
- Documentation: Followed regulatory requirements to develop daily reports and document findings into laboratory information systems
- Analysed data gathered from junior research associates.
- Performed routine lab maintenance tasks.
Consultant Research Scientist
Sri Ramakrishna Hospital
Coimbatore, Tamil Nadu
07.2016 - 07.2018
- Project Design: Addressed the issues related to oncology and other topics of interest to the hospital from proteomics and epigenetics perspective with the setup of new lab
- Publication: Successfully published two research articles based on the proposed projects and preliminary data.
- Streamlined research processes to meet tight deadlines for multiple projects.
- Provided qualified input and development support for molecular biology related research plans and study protocols.
- Worked as member of integrated project team in highly collaborative work environment.
- Conferred with scientists, engineers or others to plan or review projects or to provide technical assistance.
- Applied theories and methods of life sciences to interpret and perform analyses of experimental results.
- Detailed study findings and updated research upon new discoveries.
- Reviewed technical and professional publications and journals to stay current on recent literature and make more strategic research decisions.
Research Scientist
Sunshine Hospital
HYDERABAD, TELANGANA
12.2013 - 06.2016
- Design research protocols in collaboration with the principal investigator and other collaborators
- Performed research into study topics to increase knowledge and to provide valuable contributions.
- Applied theories and methods of life sciences to interpret and perform analyses of experimental results.
- Generated data models and performed analysis to produce reports outlining results.
- Worked as member of integrated project team in highly collaborative work environment.
- Utilized expertise in microbiology, chemistry, and hematology to help with diagnosis of medical issues.
- Reviewed technical and professional publications and journals to stay current on recent literature and make more strategic research decisions.
- Streamlined research processes to meet tight deadlines for multiple projects.
- Project Design: Addressed the problem of degenerative disc diseases and other diseases of bone from proteomics and epigenetics perspective, in collaboration with Centre for Cellular and Molecular Biology, Hyderabad
- Was head of the Molecular biology Lab consisting a team of four research fellows
- Lab Set-up: Worked without boundaries and designation to set-up a proper lab from scratch
- Result Publication: Published the results of one of the project dealing with degenerative disc disease in Spine J In 2016.
Senior Research Fellow
Basavatarakam Indo-American Cancer Hospital and Research Institute
HYDERABAD, TELANGANA
08.2012 - 06.2013
- Assist senior researchers in executing, conducting analysis and reporting results
- Design research protocols in collaboration with the principal investigator
- Experiments: Recombinant DNA Technology / Cloning, Mammalian Tissue Culture, and Sequence Analysis Tools
- Project: Role of gene regulatory mechanisms and contribution of chromatin domain boundaries in cancers
- One such mechanism contains presence of CTCF binding sites (CBS) in the near vicinity of heterochromatin
- Observation: T-cadherin (CDH13), a member of cadherin super family, a known tumour suppressor has been shown to be hyper methylated and silenced in many types of cancers
- Reserved view of the CDH13 expression status in cancer, it was hypothesized that the gene expression may be regulated by CTCF binding
- Bioinformatics: Tested hypothesis, investigated for the possible presence of CBS in the near vicinity of CDH13 gene taking a bioinformatics approach.
Junior / Senior Research Fellow
Centre for Cellular and Molecular Biology
HYDERABAD, TELANGANA
11.2001 - 05.2012
- Experiments: Recombinant DNA Technology/Cloning, Mammalian Tissue Culture, Fly Genetics, and Sequence Analysis Tools (Bioinformatics)
- PhD Enrolment: Cleared the process towards enrolment for Ph.D
- In March 2002, selected for Ph.D & enrolled for the same In Jan 2003
- Ph.D Thesis: 'Chromatin domain boundary elements that demarcate Heterochromatin-Euchromatin transition', Submitted thesis for the award of Ph.D. Submitted my thesis in February 2011, Awarded with Ph.D Degree in May 2012
- Ph.D completion under the direct guidance of Dr Rakesh Mishra, and Dr Lalji Singh Ex- Director CCMB.
- Maintained high levels of confidentiality to protect data quality and project research.
- Followed best practices and scientific protocol to reach defensible conclusions based on solid evidence.
- Upheld all university and regulatory requirements for documentation of findings.
- Recorded data from research to allow for accurate analysis.
- Used Bioinformatics, molecular cloning, Fly genetics , Mammalian tissue culture methods of investigation to research in chromatin biology topics in depth.
Education
Doctorate - Molecular Biology/ Biotechnology
Jawaharlal Nehru University
MSc - Bio-Medical Genetics
University of Madras
BSc - Zoology
Bharathidasan University
Diploma - Software Engineering
APTECH computer Education
Skills
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Certification
Introduction to the Patent Cooperation Treaty, WIPO, World Intellectual Property Organisation, June 2018
Other Information
Nominated by academic council of Bishop Heber College, Trichy as a representative of Alumni to the Board of Studies of MSc Molecular Biosciences, 2007-2008.
Key Interest Areas
- Biotechnology
- Bio-Medical Research
- Project Management
- Molecular Biology
- Cell Culture
- Proteomics
- Genetics
- Genetic Counselling
- Pain Research
- Epigenetics
- Genomics
- Degenerative Disc Diseases
- LAMP PCR
- QPCR
- Cytogenetics
Brief Summary
Through CCMB, I earned a PhD in molecular biology from JNU New Delhi. I am a flexible team player with a background in medical research, basic research, and software development. I can adapt to any system or model of research as I come from a basic sciences background. As Head Molecular Biologist, I lead a team of Six PhD holders in various fields of molecular biology. I have excellent interpersonal, communication, and relationship-building abilities. I am willing to work as a key player in technical challenging & creative environment.
Carrier Highlights
- Extremely knowledgeable in overall laboratory function and maintenance for over 11 years in Biotechnology. Experience in cell-based bioassay, various ELISAs, gel electrophoresis, qPCR. Revise and write Standard Operating Procedure (SOP). Edit and Create Standard Operating Procedures (SOPs)
- Proven experience in designing projects in the areas of basic sciences and applied sciences for the hospitals (R&D) related to the areas of interest to the hospital (Degenerative disc diseases, pain research and molecular genetics etc.)
- Extensive research experience with molecular and cellular biology techniques
- Innovative and critical thinking
- Excellent time management and organizational skills
- Strong communication skills, both written and oral
- Exercises independent judgment within defined procedures and practices to determine appropriate actions
- Participates in multifunctional teams and lead small projects
- Problem solves using knowledge of technical product details, design control and basic regulatory issues
- Designs and conducts experiments independently and writes procedures, protocols and reports
- Instrumental in designing various cloning experiments and plasmids
- Cloning, sub cloning, Oligonucleotide design, PCR, restriction digestion, gel electrophoresis, DNA purification, ligations, chemical transformation, plasmid extractions and purification, nucleic acid quantifications via spectroscopy, RNA isolation, EMSA (Electrophoretic Mobility Shift Assay), colony hybridization
- Hands on experience in mammalian tissue culture; transient transfection, creating stable cell lines, transfections using lipofection
- Performing protein quantification of SF9insectprotein, Heat shock proteins, HP1 protein etc. using ELISA
- Familiarity with literature and internal best practices to identify opportunities to improve productivity and quality of work
- Plan and execute scientific experiments related to one or more programs
- A passion for working at the bench
- A commitment to make products that solve real problems
- Actively engaged in creating and establishing well equipped laboratories, ordered and approved laboratory equipment and chemicals.
- Planned and designed all related curriculum and course materials for students and facilitated the delivery of curriculum and other educational programs.
Awards And Fellowships
- GATE 2000 in Life Sciences
- Qualified for UGC CSIR NET in life sciences 3 times (Twice awarded with JRF i.e. in CSIR UGC JRF NET exams conducted in June 2000 and Dec 2000 and qualified NET alone in DEC 1999)
- 19th Rank Holder in Bharathidasan University in BSc (Zoology) Degree examination in 1996
Poster Presentations
- Role of chromatin domain boundary elements in CDH13 gene and cancer at 8th National Research Scholars Meet in Life Sciences 2012, ACTREC Navi Mumbai
- DNA sequence elements that mark the Heterochromatin-Euchromatin Transition regions in the human genome at 4th meeting of the Asian forum of chromosome and chromatin biology 2012, CSIR- Centre for Cellular and Molecular Biology, Hyderabad
- Chromatin boundaries that separate heterochromatin and euchromatin in human genome at International symposium on Epigenetic modifications of the genome: Mechanisms and Implications 2009
- Chromatin boundaries that separate heterochromatin and euchromatin in human genome at International symposium on Nuclear Architecture and Chromatin Dynamics 2008
- Comparative genomics of the vertebrate HOX complexes at International symposium on chromosomes to genome 2007
- Chromatin boundaries in the heterochromatin–euchromatin transition zones of human chromosomes at Annual conference of Indian society of Developmental Biologists and International symposium on Cellular signalling during Development 2006
- Comparative genomics of the vertebrate HOX complexes at EMBO international workshop on Upstream and Downstream of Hox Genes 2005
- Functional implication of genomic organization: the role of non-coding DNA at 8th ADNAT symposium on comparative and functional genomics 2004
- Genome organization, higher order chromatin structure and developmental gene regulation at CCMB silver jubilee symposium on the current excitement in Biology 2002.
- Functional genomics of non- coding DNA: Transgenic approach at 6th ADNAT symposium on functional genomics 2002
Invited Presentations
- Role of Chromatin Domain Boundaries in Cancer International symposium on Computational Biology and Drug Design July 2013 MACFAST, Tiruvalla, Kerala, India
- Chromatin Domain Boundaries in Cancer National seminar on Trends in Biological Sciences March 2013, Bishop Heber College Trichy
- Epigenetics of pain 32nd Annual National Conference of Indian Society for the study of pain February 2017
Research
10+ Years
Publications
Quantitative proteomic analysis of normal and degenerated human intervertebral disc. Spine J. 2016 Aug; 16(8):989-1000 Comparative Genomics of Myocilin protein. Int J Pharm Bio Sci 2015 July; 6(3): (B) 992 – 1005. Sivaraman K, Vijayanand P. Epigenetics, pain, and analgesia. Indian J Pain 2017; 31:7-8 DOI: 10.4103/ijpn.ijpn_30_17 Dhaulakhandi DB, Krishnan S (2017) Are Lymphomas Result of Aberrant Epigenentic Regulation of Immune System Molecular Insights and Therapeutic Prospects. MOJ Lymphol Phlebol 1(1): 00002. DOI:10.15406/mojlp.2017.01.00002 Krishnan Sivaraman. Epigenetics and Pain. More nurture than nature 15 April 2014. Available from: http://travelingpainschool.com/epigenetics-and-pain-more-nurture-than-nature/challenges. (Last accessed on 2017 May 02) Manuscripts under preparation: S Krishnan, Shreekant Verma and Rakesh K Mishra. Sequence motifs that mark the transition regions between hetrochromatin and euchromatin in human genome “Stem cell therapy for pancreatitis and translational evaluation of prospective experimental strategies for regeneration of exocrine pancreas” Dhaulakhandi DB, and Krishnan S Krishnan S. Role of chromatin domain boundary elements in CDH13 gene and cancer
Timeline
Head R&D / Consultant Scientist
BioArtis Life Sciences/Sentier Consulting & Healthcare
2023.07 - Current
Head – Molecular Biologist
Genes2me
02.2022 - 06.2023
Solutions leader (Subject matter expert (Molecular biology))
Brane Enterprises private limited
12.2019 - 12.2021
Research Scientist
Bioartis life sciences private Ltd
02.2019 - 12.2019
Molecular Biology Research and Development
GCC Biotech
09.2018 - 02.2019
Consultant Research Scientist
Sri Ramakrishna Hospital
07.2016 - 07.2018
Research Scientist
Sunshine Hospital
12.2013 - 06.2016
Senior Research Fellow
Basavatarakam Indo-American Cancer Hospital and Research Institute
08.2012 - 06.2013
Junior / Senior Research Fellow
Centre for Cellular and Molecular Biology
11.2001 - 05.2012
Doctorate - Molecular Biology/ Biotechnology
Jawaharlal Nehru University
MSc - Bio-Medical Genetics
University of Madras
BSc - Zoology
Bharathidasan University
Diploma - Software Engineering
APTECH computer Education
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