Surabhi Agnihotri

Analytical researcher with experience in drug-designing research studies and developing countermeasures against targeted diseases. Proficient in drug design and development, cell culture, and protein biochemistry. Skilled in performing bioassays, laboratory bench studies, writing scientific reports, and maintaining research lab operations and equipment.

• Received First Prize (2024) Oral presentation in 39th M.P. Young Scientist Congress 2024 held at MITS, Gwalior.
• Received Third Prize (2022) - e-short presentation in International Conference on Advances in Biosciences and Biotechnology (ICABB-2022) held at Jaypee Institute of Information Technology, Noida, U.P
• Awarded by MHRD GATE Fellowship 2019-2022.
• Awarded by DBT- Biotech Industrial Training Programme 2016-2017.

• Papers presented:
• Agnihotri S. “One-pot multi-step method to synthesize novel 2-[(8-hydroxyquinolin-7 yl)(phenyl)methylamino]benzoic acid derivatives and their biological evaluation”. Oral presentation in 39th MP Young Scientist Congress 2024 organized by MPCST, Bhopal at MITS, Gwalior during February 21st 23rd, 2024.
• Agnihotri S. “Identification of 8-Hydroxychloroquinoline Derivatives as Common Potent Inhibitor/s of the Most Poisonous Toxin: Design, Molecular Docking Study and Synthesis”. Poster presentation at International Conference on Drug Discovery 2022 at BITS-Pilani, Goa during November 10th-11th, 2022.
• Agnihotri S. “In-silico Modeling and Identification of Common Potent Inhibitor/s Against the Most Poisonous Toxins”. E-short presentation at International Conference on Advances in Biosciences and Biotechnology 2022 at Jaypee Institute of Information Technology during January 20th – 22nd, 2022.
• Attended 25th INDO-US Flow Cytometry Workshop held at CSIR-CDRI, Lucknow on 7th-8th Feb 2024. Organized by CSIR-Central Drug Research Institute (CSIR-CDRI), Lucknow, India & Trust for Education and Training in Cytometry (TETC), India
• Industrial visit at Serum Research Institute, Pune (2016).

• Agnihotri, S., Kushwah, V.C., Bansal, S. et al. 2024. Novel 2-[(8-hydroxyquinolin-7-yl)(phenyl)methylamino]benzoic acid analogs targeting the active site of botulinum neurotoxins: designing, synthesis, and biological evaluation. Med Chem Res 33, 1625–1642. (IF 2.6)
• Chauhan R., Chauhan V., Agnihotri S., Vimal M., Saxena N., Dhaked R.K., 2022. Designing, synthesis and evaluation of derived analogues of selected small molecule non-peptidic inhibitors against serotype BoNT/ F. Toxicon 222: 106981. (IF 2.8)

• Anaerobic media preparation and production of toxin.
• Cloning, expression of various proteins in prokaryotic system and protein purification.
• Performed wet-lab and molecular biology scientific research using techniques like agarose gel electrophoresis PCR, Gradient PCR, RT-PCR SDS- PAGE, western blot analysis, endopeptidase assay and chemiluminscence assay, etc.
• Biological activity assay of proteins, development of HTS assay for screening inhibitors using FRET based, fluorescence thermal shift, and surface plasmon resonance assays, etc.
• In silico screening of the compounds against the targeted proteins. Pubmed searching, FASTA, BLAST, molecular docking using Schrodinger Maestro, AutoDock 4.2, Molegro Virtual Docker, and molecular dynamic simulation study.
• Expertise in cell culture experiments (A549, Vero cells, etc.) like maintenance of cell lines, cell viability assay viz., MTT, neutral red dye uptake assay and LDH release assay etc.
• Evaluation of small non-peptidic molecule-based therapeutics and toxicity of botulinum neurotoxin in mouse model.
• Antisera production and purification of IgG antibodies.
• Development of Indirect and Sandwich ELISA.
• Plant tissue culture techniques.

1. Dr. Ram Kumar Dhaked   Scientist 'G' & Head,   

Biotechnology Division  

Defence Research & Development Establishment (DRDE-DRDO)

Jhansi Road, Gwalior- 474002, Madhya Pradesh, India

Tel: 0751-2390274 (O);+91 9425123163 (R)

Email: ramkumardhaked@hotmail.com

2. Dr. Nandita Saxena, Scientist ‘F’  

Pharmacology and Toxicology Division  

Defence Research & Development Establishment (DRDE-DRDO)  

Jhansi Road, Gwalior- 474002, Madhya Pradesh, India  

Tel: 0751-2390366 (O)

Email: saxenanandita@gmail.com

3. Dr. Manorama Vimal, Scientist 'E'

Test and Evaluation Division,

Defence Research & Development Establishment (DRDE-DRDO) 

Jhansi Road, Gwalior- 474002, Madhya Pradesh, India  

Tel: 0751-2390171 (O); +91 7489470725 (R)  

Email: saxenanandita@gmail.com