Summary
Overview
Work History
Education
Skills
Affiliations
Languages
Interest
Declaration
Timeline
Generic

SWAPNIL GAIKWAD

Pune

Summary

As an officer at Lupin Limited, I develop and optimize protein extraction and purification methods for recombinant proteins, using downstream filtration and chromatographic techniques. I also perform refolding, viral filtration, CIP, SIP, and integrity testing of downstream production equipment. I have experience in working with pharmaceutical software such as Caliber QAMS, SAP, eDMS, DMS, L2LMS, e-log, SABA, and ARIBA, and in preparing, revising, and reviewing SOPs, change control, and CAPA. I have a master's degree in biotechnology from Dr. Babasaheb Ambedkar Marathwada University, where I learned the principles and applications of biotechnology in various fields. I have also worked as a scientific officer at Krsnaa Diagnostics, where I performed COVID-19 viral RNA and antigen tests, and as a junior officer in R&D at Hi-Tech Biosciences, where I worked on probiotics and biocatalysts. I am skilled in R&D microbiology, GMP compliance, and quality risk management. I am motivated by the challenges and opportunities in the biopharmaceutical industry, and the potential to improve human health and well-being.

Overview

7
7
years of professional experience

Work History

Officer

Lupin Ltd (Biotechnology division)
Pune
01.2021 - Current
  • Mammalian downstream: Development of protein extraction and purification methods for recombinant proteins using downstream filtration and chromatographic techniques, well served with affinity, HIC, AEX, and mix-mode chromatography.
  • Refolding of the protein process.
    Concentration and buffer exchange using the TFF system.
  • Viral Filtration (nanofiltration).
  • Cleaning and sterilization of downstream production equipment (CIP and SIP).
  • Integrity testing of liquid filters.
  • Column packing and evaluation. Handy in working on pharmaceutical software such as QAMS, SAP, eDMS, DMS, L2LMS, e-log, SABA, SPARK, ARIBA, etc.
  • Experienced in the preparation, revision, and review of SOPs, change control, and CAPA.
    Preparation of the impact assessment report.
  • Handy soft working experience in AKTA System Process. (Version: Unicorn 7.6.)

Scientific Officer

Krsnaa Diagnostics Pvt. Ltd
Pune
09.2020 - 12.2020
  • COVID-19 viral RNA qualitative test.
  • COVID-19 Rapid Antigen Test.
  • Validation of emerged RT-PCR and extraction kits.
  • I design templates and SOPs as per ISO 15189:2012.
  • Technologies used: Thermo Fisher Scientific, PerkinElmer, Abbott, Lab Systems, MyLab.
  • Skills: RNA, DNA Extraction, PCR (Version: QUANT 5, QUANT 6).

Junior Officer (R&D Molecular Biology)

Hi Tech Biosciences India Ltd.
Pune
01.2019 - 06.2020
  • Worked on probiotics and biocatalysts on Mycobacterium smegmatis, E. coli, transaminase, nitrilase, and CYP450 enzymes.
  • Isolation of bacterial plasmid DNA and its qualification.
  • Transformation of competent E. coli cells with recombinant plasmid DNA.
  • Preparation and validation of glycerol stocks of recombinant E. coli strains and E. coli clones.
    Induction of recombinant cells with IPTG.
  • Small-scale screening of E. coli clones for protein expression.
  • Preparation of microbiological media, reagents, and buffers.
  • Preparation of carbenicillin, kanamycin, tetracycline, chloramphenicol, and hygromycin antibiotic stock.
  • Optimize inoculum for their size, age, temperature, pH, and other growth conditions by using a growth curve.
  • Calibration of micropipettes, weighing balance, pH meter, water bath, and refrigerator of the laboratory.
  • Data analysis, documentation, results, report preparation, and data presentation.

Trainee

Wockhardt Ltd. (Biotech Park)
Aurangabad
07.2018 - 01.2019

Education

M.Sc Biotechnology - Life Sciences

Dr. BAMU Sub Center
Osmanabad, India
06-2018

B.Sc Biotechnology - Life Sciences

Lokmangal Biotechnology College,
Solapur,India
05-2016

Skills

  • Protein purification
  • Chromatography techniques
  • Analytical R&D
  • Effective communication
  • Problem solving
  • Regulatory compliance
  • Downstream processing
  • RT-PCR
  • Laboratory equipment maintenance
  • Computer system validation
  • Microscopy
  • Sonication

Affiliations

  • Participated in the 'Poster Presentation and Article Writing Competition' held on January 20, 2016 Organized by Walchand college of Art & Science, Solapur.
  • Participated in "National level puls Polio campaign" Tuljapur.
  • Completed Service course on "Personality Development" at 3rd sem. of M.Sc Held on July-Nov 2017.
  • Participated in "N.S.S campaign" programmed at Osmanabad.
  • Attended workshop on "Industrial Technology" at Department of Microbiology Dr. BAMU Sub-campus Osmanabad.

Languages

Marathi
Native
Native
Hindi
Proficient (C2)
C2
English
Proficient (C2)
C2

Interest

  • Reading, Writing, Singing, Social activities, Playing Cricket, Volleyball, Chess

Declaration

  • I do hereby declare that the above information is true.

Timeline

Officer

Lupin Ltd (Biotechnology division)
01.2021 - Current

Scientific Officer

Krsnaa Diagnostics Pvt. Ltd
09.2020 - 12.2020

Junior Officer (R&D Molecular Biology)

Hi Tech Biosciences India Ltd.
01.2019 - 06.2020

Trainee

Wockhardt Ltd. (Biotech Park)
07.2018 - 01.2019

M.Sc Biotechnology - Life Sciences

Dr. BAMU Sub Center

B.Sc Biotechnology - Life Sciences

Lokmangal Biotechnology College,
SWAPNIL GAIKWAD