VINAY BHARDWAJ
REWARI,HARYANA
Summary
Protein biochemistry, and custom-built in-vitro experimental systems. Trained in working at low signal-to-noise limits, handling purified proteins, and building reproducible measurement pipelines where Experimental researcher with hands-on experience in single-molecule fluorescence imaging, experimental stability and surface effects critically determine data quality. Seeking PhD training in single-molecule biophysics at the interface of optics, electric fields, and bio-molecular interactions.
Overview
3
3
years of professional experience
Work History
Research Intern
IonCure
05.2025 - Current
- Analyzed molecular interaction networks using Python
- Built quantitative data analysis and visualization pipelines
- Strengthened statistical reasoning and reproducible analysis practices
- Developed strong habits in data integrity and automation
Nanobody Based DNA-PAINT Probe Development
Indian Institute of Science
Bengalore
05.2023 - 05.2024
- Developed fluorogenic DNA-PAINT probes for quantitative single-molecule localization microscopy
- Worked extensively with purified proteins and nanobody–protein conjugates
- Performed mammalian cell culture, immunocytochemistry, and fluorescence imaging
- Acquired large single-molecule datasets where optical stability, background suppression, and surface effects directly limited measurement quality
- Built Python-based pipelines for localization analysis and quantitative interpretation
- Gained experience working at the physical limits of optical detection, where small experimental instabilities significantly affected outcomes
Oral Vaccine Development Project
IISER Tirupati
Tirupati
01.2023 - 04.2023
- Designed and fabricated microfluidic platforms for controlled temporal perturbations
- Integrated optical readouts with fluidic control systems
- Developed stable experimental workflows for reproducible measurements
- Gained experience interfacing physical systems with biological samples
Education
Integrated BS-MS - Biological Sciences
Indian Institute of Science Education and Research (IISER) Tirupati
Tirupati05.2024
Skills
Optics & Imaging
Fluorescence microscopy
Super-resolution microscopy (DNA-PAINT, SMLM concepts)
Time-lapse and quantitative imaging
Image noise reduction and signal optimization
Protein & Biochemistry
Recombinant protein expression and purification
Affinity chromatography, SDS-PAGE, Western blot
Protein labeling and conjugation chemistry
Handling low-concentration protein samples
Surface & Interfaces
References
- Sukant, Khurana, Dr., sk@ioncurex.com, Univ. of Colorado / IonCure Group
- Dileep, Mampallil, Dr., dileep.mampallil@iisertirupati.ac.in, IISER Tirupati
- Alfredo, Ghezzi, Dr., alfredo@ioncurex.com, Univ. of Puerto Rico
- Pakala Suresh, Babu, Prof., pakalasb@uohyd.ac.in, University of Hyderabad
- Brooks, Robinson, Dr., brobinson@ioncurex.com, Univ. of Colorado
RESEARCH INTERESTS
- Single-molecule biophysics and protein-scale measurements
- Optical detection at low signal-to-noise limits
- Electric-field interactions with biomolecules
- Protein–surface interactions in confined environments
- Quantitative experimental methods at the bio–physical interface
Timeline
Research Intern
IonCure
05.2025 - Current
Nanobody Based DNA-PAINT Probe Development
Indian Institute of Science
05.2023 - 05.2024
Oral Vaccine Development Project
IISER Tirupati
01.2023 - 04.2023
Integrated BS-MS - Biological Sciences
Indian Institute of Science Education and Research (IISER) Tirupati