Summary
Overview
Work History
Education
Skills
Extracurricular Activities
Timeline
Generic

Pratiksha Tolmare

Pune

Summary

Junior research fellow with a passion for advancing knowledge and contributing to groundbreaking research initiatives. Skilled in utilizing research methodologies and tools to support project objectives. Strong academic background and keen analytical abilities, coupled with a proactive approach to problem-solving.

Overview

3
3
years of professional experience

Work History

Junior Research Fellow

Reliance Life Science Pvt. Ltd.
Pune
09.2022 - Current
  • Peg G Method development for secondary structure estimation by Circular dichroism spectroscopy (CD) and Fourier-Transform Infrared Spectroscopy (FTIR)
  • Method development and Qualification for tertiary structure analysis by fluorescence spectrophotometer.
  • Method Development on Nano DSC Instrument to determine the Tm of different Monoclonal Antibodies and recombinant protein.
  • Worked on determination of Higher Order Structure for Pegfilgrastim, Nivolumab, Golimumab, and Aflibercept by using FTIR, CD-Spec, fluorescence spec, and DSC.
  • Stability analysis of Abatacept, Golimumab, and Natalizumab.
  • Invert Stability of Abciximab, Bevacizimab, and Rituximab.
  • Hold Time Study of Omalizumab.
  • Free Cysteine Assay, Protein A ELISA, and Bacterial Endotoxin Test analysis.

Genetics Lab Technician

Bharati Hospital & Research Center
Pune
04.2022 - 08.2022
  • Worked on fluorescent in-situ hybridization patient suffering from acute lymphoid leukemia (Cancer).

Intern

Interactive Research School for Health Affairs (IRSHA)
Pune
09.2021 - 03.2022
  • Worked on research project entitled, 'Effect of Yoga intervention on skeletal muscle linked glucose homeostasis in pre-diabetic individuals'
  • Performed the synthesis of PHBV nanoparticle and its characterization - Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by various bacterial species
  • Characterization of PHBV done by SEM (scanning electron microscopy) at National Chemical Laboratory, Pune.

Education

Masters of Science in Biotechnology -

Bharati Vidhyapeeth Deemed University
Pune, Maharashtra, India
07.2021

Bachelor of Science in Biotechnology -

Savitribai Phule Pune University
Maharashtra, India
04.2019

Maharashtra Higher Secondary School Certificate Examination (12th) -

H. V. Desai College of Arts and Science
05.2016

Maharashtra Secondary School Certificate Examination (10th) -

Samajbhushan Baburao Phule Madhyamik Vidyalay
05.2014

Skills

    Well proficient in handling and calibrating Higher Order Structure instruments like Nano-DSC, CD-Spectroscopy, FTIR and FP-Spectroscopy

    Hands-on experience in handling and calibration of High Performance Liquid Chromatography along with knowledge of different columns used to perform various types of chromatography

    Well verse use of instrument software’s like LabSolutions, Chromeleon 72, Labware LIMS and Document Management System

    Complete understanding of various characterization related instrument software’s like

    FP- Spectra Manager Ver2 CFR

    FTIR- Spectra Manager Ver21502 CFR

    CD- Spectra Manager Ver21502 CFR

    NANO DSC (DSCRun and NanoAnalyze)

Extracurricular Activities

Personality Assessment award at District level (2nd place prize) Student of the year award (10th Std) Participated in dodge ball at inter campus level Participated in Mallakhamb (rope) at inter school level Participated and won several awards in various dance competitions

Timeline

Junior Research Fellow

Reliance Life Science Pvt. Ltd.
09.2022 - Current

Genetics Lab Technician

Bharati Hospital & Research Center
04.2022 - 08.2022

Intern

Interactive Research School for Health Affairs (IRSHA)
09.2021 - 03.2022

Masters of Science in Biotechnology -

Bharati Vidhyapeeth Deemed University

Bachelor of Science in Biotechnology -

Savitribai Phule Pune University

Maharashtra Higher Secondary School Certificate Examination (12th) -

H. V. Desai College of Arts and Science

Maharashtra Secondary School Certificate Examination (10th) -

Samajbhushan Baburao Phule Madhyamik Vidyalay

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Pratiksha Tolmare