Ravi Teja Ayyagari

Led end-to-end lab design, including resource planning, procurement, and qualification of cell culture equipment.

• Project lead for early stage process development which involves clone screening and process development across different scales including Ambr15, ambr250, 5L and 50L.
• Applied QbD and statistical methods to design experiments (DoE) aimed at optimizing the afucosylation, charge profiles, and aggregation of biologics during early-stage development.
• Developed N-1 perfusion models for scale up.
• Used coordination and planning skills to achieve results according to schedule.
• Used critical thinking to break down problems, evaluate solutions and make decisions.
• Thoroughly completed all necessary paperwork, consistently identifying discrepancies and promptly addressing them to ensure swift resolution.
• Proficient in using electronic laboratory notebooks (ELNs) to document experiments and maintain accurate records.

• Led end-to-end lab design, including resource planning, procurement, and qualification of cell culture equipment.
• Conducted lab scale process development, scale-up, and tech transfer to manufacturing for the production of mabs and therapeutic proteins.
• Conducted pre-clinical and clinical process development and process transfer into cGMP.
• Conducted root cause analyses (RCAs), risk assessments, Design of Experiments (DoE), multivariate analysis, and Failure Mode and Effects Analysis (FMEA) to optimize manufacturing processes.
• Developed scale-down models and conducted process characterization studies to optimize manufacturing processes.
• Analyzed and interpreted manufacturing data to evaluate process performance.
• Coordinated, planned, and supported the execution of manufacturing process validation runs.
• Authored and reviewed comprehensive manufacturing and technology transfer protocols, as well as reports summarizing studies and projects.
• Skilled in coaching and mentoring junior scientists while also monitoring technical performance across the network.

• Played a key role in technology transfer and drafting process protocols, while also driving process optimization efforts in the lab and supporting the transfer of optimized processes to manufacturing.
• Gap analysis and resource management. Optimized process conditions to meet client's requirements.
• Gathered, arranged and corrected research data to create representative graphs and charts highlighting results for presentations.
• Streamlined research processes to meet tight deadlines for multiple projects.
• Helped team meet regulatory requirements by coordinating documentation and filings.

• As an integral member of a team, contributed to the development, optimization, and scale-up of the process and feed parameters for the production of recombinant monoclonal antibodies and proteins (biosimilars) in CHO cells.
• As an individual contributor, successfully optimized and established processes in single-use bioreactors, achieving target results. This included optimization of glycan and charge variants.
• Identified issues, conducted data analysis, and provided effective solutions to problems.
• Proficiently utilized Microsoft Excel to create spreadsheets for daily, weekly, and monthly reporting.