Summary
Overview
Work History
Education
Skills
Websites
Certification
Accomplishments
Publications
References
Conferences & Presentations
Timeline
Generic

Rohini Krishna Kota

Executive-L1 at Bharat Biotech International Limited
Hyderabad

Summary

Highly motivated researcher with experience of 4 years which has nurtured my scientific temperament in designing and developing various immunoassays, molecular cloning in prokaryotic system, immunizing mouse and chicken models, antibody purification, CRISPR-mediated genome editing. Performed cell-based assays to support research in the field of mitochondrial biology. Authored peer reviewed scientific publications which highlight novel research outcomes. Strong propensity to work in teams and manage challenging scenarios to ensure timely project execution. Effective team member with excellent written/oral communication skills and the ability to summarize research findings. Seeking to leverage acquired scientific competence and apply ingenuity through independent research as well as collaborate with interdisciplinary research teams to develop and test translational applications of research findings.

Overview

5
5
years of professional experience
9
9
years of post-secondary education
1
1
Certification

Work History

Executive L1

Bharat Biotech International Limited
06.2023 - Current
  • Evaluating the immunogenicity of vaccines and evaluating pre-clinical and clinical samples.
  • Preparation of STPs and SOPs.
  • Actively involved in method development, troubleshooting various experimental parameters and finding better approaches to reach the target.


Senior Research Associate

Dr. Reddy's Institute of Life Sciences
02.2022 - 05.2023
  • Established working protocols to generate knockout of gene targets using CRISPR technology and expanded multiple clones to verify CRISPR knockout
  • Understanding the cellular and molecular basis of mitochondrial biology
  • Achieved project objectives by troubleshooting experiments and testing practical and creative solutions
  • Handling collaborative assignments of clinical significance
  • Co-ordination of lab maintenance.

Senior Research Associate

Loka Biosciences Pvt. Ltd
04.2021 - 02.2022
  • Cloned and expressed spike protein of SARS-CoV2
  • Tested the expression of spike protein construct in HEK293T cells
  • Co-ordination of lab maintenance.

CSIR-Senior Research Fellow

Vignan's Foundation For Science Technology and Research
04.2019 - 03.2021
  • Development and Evaluation of Cross-reaction Free Diagnostics against Staphylococcus aureus.

Education

Ph.D. - Biotechnology

Vignan's University
Guntur, Andhra Pradesh
07.2017 - 05.2021

M.Tech - Biotechnology

Vignan University
Guntur, Andhra Pradesh
07.2015 - 05.2017

B.Tech - Biotechnology

Vignan University
Guntur, Andhra Pradesh
08.2011 - 05.2015

Skills

Mammalian gene expression analysis (Quantitative Real time PCR)undefined

Certification

Workshop on Laboratory Animal Handling and Research, Center for Laboratory Animal Technology and Research, Sathyabama Institute of Science and Technology, Chennai, 8 - 10th May, 2019

Accomplishments

  • Awarded CSIR Direct - SRF, Jan 2019-Mar 2021
  • Honored with the Gold medal and Young Scientist Award, International Conference on Environmental Sustainability, Human Health and Development and 13th Annual Convention of Association of Biotechnology and Pharmacy, 20 - 22nd December, 2019
  • Received Best Distinguished Alumni honor from Vignan's Foundation for Science, Technology and Research University

Publications


  • Immunoinformatics analysis and evaluation of recombinant chimeric triple antigen toxoid (r-HAB) against Staphylococcus aureus toxaemia in mouse model, Applied Microbiology and Biotechnology, Kota, R. K., Kolla, H. B., Reddy, P. N., Kalagatur, N. K., & Samudrala, S. K., 2021, 1-15, 4.813
  • Application of IgY antibodies against staphylococcal protein A (SpA) of Staphylococcus aureus for detection and prophylactic functions, Applied Microbiology and Biotechnology, Kota, R. K., Reddy, P. N., & Sreerama, K., 2020, 104, 21, 9387-9398, 4.813
  • IgY antibodies of chicken do not bind staphylococcal binder of immunoglobulin (Sbi) from Staphylococcus aureus, Annals of Microbiology, Kota, R. K., Srirama, K., & Reddy, P. N., 2019, 69, 5, 531-540, 2.7
  • Recent advances in probiotics as live biotherapeutics against gastrointestinal diseases, Current pharmaceutical design, Kota, R. K., Ambati, R. R., YVV, A. K., Srirama, K., & Reddy, P. N., 2018, 24, 27, 3162-3171, 3.116
  • Functional characterization of a broad and potent neutralizing monoclonal antibody directed against outer membrane protein (OMP) of Salmonella typhimurium, Applied microbiology and biotechnology, Reddy, P. N., Makam, S. S., Kota, R. K., Yatung, G., Urs, R. M., Batra, H., & Tuteja, U., 2020, 104, 6, 2651-2661, 4.813
  • Purification and lignocellulolytic potential of cellulase from newly isolated Acinetobacter indicus KTCV2 strain, Iranian Journal of Science and Technology, Transactions A: Science, Karlapudi, A. P., Venkateswarulu, T. C., Srirama, K., Dirisala, V. R., Kamarajugadda, B. P., Kota, R. K., & Kodali, V. P., 2019, 43, 3, 755-761, 1.19

References

  • Dr. Kishore Parsa, Principal Res Scientist, Dr. Reddy's Institute of Life Sciences, Hyderabad, Telangana, 500046, KishoreP@drils.org
  • Dr. Aarti Sevilimedu, Principal Res Scientist, Dr. Reddy's Institute of Life Sciences, Hyderabad, Telangana, 500046, AartiS@drils.org
  • Dr. S. Krupanidhi, Professor, Vignan's Foundation for Science, Technology and Research University, Guntur, Andhra Pradesh, 522213, krupanidhi.srirama@gmail.com
  • Dr. Satyanarayana N. Gummadi, Professor, Department of Biotechnology, IIT-Madras, Tamilnadu, 600036, gummadi@iitm.ac.in

Conferences & Presentations

  • IgY: A Promising Alternative to IgG in Immunodiagnostics, International Conference on Environmental Sustainability, Human Health and Development and 13th Annual Convention of Association of Biotechnology and Pharmacy, 20 - 22nd December, 2019, Oral Presentation
  • A concept note on IgY based Immuno-PCR for Sensitive Detection of COVID-19, International E-conference on Recent Trends in Drug Discovery, Diagnostics and Therapeutics. A special emphasis on COVID-19, July 2nd - July 4th , 2020, Oral Presentation
  • Development and Evaluation of Co-agglutination assay for Bacillary Dysentery, International conference on BIOTECHNOLOGY & BIOENGINEERING-TRENDS, 24 - 26th March, 2017, Oral Presentation
  • Potential of commonly grown Indian plants towards mosquito repellent activity, INTERNATIONAL SYMPOSIUM, ESTIEH-2014, 1-3 August 2014, Oral Presentation

Timeline

Executive L1

Bharat Biotech International Limited
06.2023 - Current

Senior Research Associate

Dr. Reddy's Institute of Life Sciences
02.2022 - 05.2023

Senior Research Associate

Loka Biosciences Pvt. Ltd
04.2021 - 02.2022

CSIR-Senior Research Fellow

Vignan's Foundation For Science Technology and Research
04.2019 - 03.2021

Ph.D. - Biotechnology

Vignan's University
07.2017 - 05.2021

M.Tech - Biotechnology

Vignan University
07.2015 - 05.2017

B.Tech - Biotechnology

Vignan University
08.2011 - 05.2015
Workshop on Laboratory Animal Handling and Research, Center for Laboratory Animal Technology and Research, Sathyabama Institute of Science and Technology, Chennai, 8 - 10th May, 2019
Rohini Krishna KotaExecutive-L1 at Bharat Biotech International Limited