SUKALPA MONDAL

• Presales technical support for multiple NGS workflows covering the whole spectrum of NEB NGS kits , Invitro Transcription, genome editing, cloning workflows and Isothermal reaction workflows.
• Incorporated NEBNext reagents in niche workflows like MeRIP, CUT&RUN, PLAC-seq and low input ChIP-seq in premium research labs like NII, IGIB, ISI.
• Onsite wet lab NGS library prep in both private and Govt institutes in North India, East India and West India. Completed onsite library preparation/training for WGS, RNA-Seq, WGS-PCR free,CUT&RUN and ultra low/single cell library preparation using NEB kits.
• Onsite Oxford Nanopore library preparation for WGS workflow in MinION Mk1B Platform.
• Technical presales and onsite support for CST workflows like CUT&RUN and CUT&Tag.
• Presales technical support for ONT platforms and workflows related to long reading sequencing.
• Primary dealer based post sales technical support and troubleshooting for all NEB related reagents and queries. Technical queries that cannot be addressed were then taken up with NEB technical team.
• Dealers and Internal Sales training.
• Onsite Technical presentation to end user for ONT and short read sequencing workflows.

• Performing various aspects of Techno-commercial, Project scoping and Technical Support in wide range of Genomics and Epigenomic applications( Eg: WGS, WGBS,RRBS-seq, cfDNA-seq, ddRAD , RNA-seq, small RNA-seq, Hi-C for insects, plant roots, mammalian cells, ATAC-seq, ultra low RNA-Seq).
• Development and establishment of SOPs for for library preparation in niche workflows like ChIP-Seq, ATAC-Seq, CUT&RUN, MeDIP-Seq, insitu Hi-C using ARIMA kit, Ribomethyl-seq and RIP-seq.·
• Keeping track of leads, providing technical support and keeping tracks of projects during both wet lab and dry lab delivery.
• Developing budgets for new and current projects.
• Analyzing QC data and SOPs, evaluate new reagents, and optimize protocols for the wet lab component of multi-omics workflows.
• Improving the company's service offering, assess the applicability of new technologies and protocols for both long and short reads.

I was working with two different model systems for my doctoral studies.

A. Neurogenesis- Monolayer differentiation of mouse embryonic stem cells to neural stem cells to cortical neurons.

B. Lactogenesis-Differentiation of Prolactin responsive HC11 cells to Casein secreting HC11 cells in presence of Insulin, Hydrocortisone and Prolactin. The primary objective of my research was to study how spatiotemporal dynamics of 3D genome orchestrates transcriptional output and preserve transcript stoichiometry. End-to-end studies

including Hi-C, ATAC-Seq, HiChIP, and Gro-Seq were planned and executed.

Publication : Comprehensive Profiling of Transcriptional Networks Specific for Lactogenic Differentiation of HC11 Mammary Epithelial Stem-like Cells. Sci Rep 8, 11777 (2018). Link: https://doi.org/10.1038/s41598-018-30122-4.

Cell culture,RNA and DNA extraction from cells. Real time PCR, Chromosome Conformation Capture(3C) and its Primer design.