SURESH PALANIVEL

• Doctoral Dissertation "Evaluation of cytotoxic effects and underlying mechanisms of phenolic compounds on breast cancer cell lines"
• Maintain cell lines and prepare cell for drug treatment
• Perform cytotoxicity assay to measure the toxicity
• Perform CASPASE assay to determine the apoptosis
• Perform AO/ETBR and FACS to study the different cell population of apoptosis
• Perform fluorescence microscopy for staining techniques
• Extract DNA/RNA samples from cell cultures.
• Perform RT-PCR to determine the gene expression
• Perform computational models such molecular docking and molecular dynamic simulation to understand the molecular interactions between the ligand and the receptors

• Research Topic "Predicting the potential drugs against breast cancer using advanced computational tools"
• Perform molecular docking to study the interactions
• Perform molecular dynamic simulations to study the stability of the interactions and the conformational changes
• Perform MMGBSA to evaluate the free binding energy of the interactions

• Research Topic "Predicting the efficacy of the Non-nucleoside Agonists at the A2A Adenosine Receptor"
• Maintain cell lines and prepare cell for drug treatment
• Perform cytotoxicity assay to measure the toxicity
• Perform RT-PCR to determine the gene expression
• Perform computational models such molecular docking and molecular dynamic simulation to understand the molecular interactions between the ligand and the receptors

Research Topic "Cross Disease Analysis of pathways related to neurodegenerative diseases"

• Perform modeling of GPR17 as a target receptor
• Perform ligand preparation
• Perform molecular docking to determine interactions between the ligand and receptor
• Perform molecular dynamic simulations to determine the stability of the interactions
• Perform transient transfection of plasmid pcDNA3.1-hGPR17
• Perform measurement of secondary messenger cAMP

Research Topic "Functional genomics and proteomics of fungal pathogenome and host immune response to fungal infection in human mycotic keratitis"

• Maintain cell lines and prepare cells for various experiments
• Perform 2D-SDS PAGE to isolate the proteins
• Perform Western Blotting to separate and identify proteins
• Perform ingel tryptic digestion for sample preparation in LC MS/MS
• Prepare patients sample for tear proteomic analysis

• Establishment of quality systems that are maintained and followed as per GMP/GLP norms
• Coordinating with all cross function teams like Administration, Engineering, Supply chain management, and etc. for the User team (Science team) requirements
• Assist in safety related work streams as per requirement
• Inventory management of chemicals, consumables etc.

Research Topic "Macrophage-mediated Lung Damage by Nano Particles"

• Perform Carbon Bulkparticles and Nanoparticles preparation
• Perform LDH Cytotoxicity Assay to determine cellular cytotoxicity
• Perform MTT assay to assess cell metabolic activity
• Perform microscopic analysis to determine the morphology of the cells

Research Topic "Isolation of endophytic fungi from a medicinal plant Guazuma tomentosa L., with special reference to anti-microbial activity and taxol production"

• Perform Isolation of Endophytic Fungi
• Perform Radial Growth Analysis of Phomopsis sp.,
• Perform extraction of Taxol
• Perform Thin Layer Chromatography
• Perform High Pressure Liquid Chromatography
• Perform Infra-Red Spectroscopy and Ultra-Violet Spectroscopy
• Perform screening of Anti-Bacterial Activity of crude extracts of Phomopsis sp.,