Mohammed Azhar
Visakhapatnam
Summary
Researcher with 19+ years of R&D experience in Biotechnology field.
- Experience in vector construction, cloning of the gene, stable cell line development and purification.
- Expertise in antibody discovery, antibody engineering and cell line engineering
- Strong capabilities in training, team development and lab management
Overview
19
19
years of professional experience
Work History
Asst. General Manager
Transasia Bio-Medical Pvt Ltd
VISAKHAPATNAM
2021.08 - Current
Head-Monoclonal antibody and Protein generation
My work responsibilities includes
- Monoclonal antibody generation through Hybridoma and Phage display technology
- Protein expression through Emboli, Pichia, Insect and mammalian expression systems
- Stable cell line development by Glutamine synthetase expression system in CHO-K1 cells
- Purification of antibodies and proteins
- Team management.
Principal Investigator
Syngene International Ltd
Bangalore
2017.07 - 2021.07
As a group leader of the protein expression group, my work responsibilities includes
- Stable cell line development for expressing different formats of mAbs in Piggyback expression system (from mg to grams level).
- Mammalian display platform establishment by Lent virus method.
Research Scientist
Lupin Ltd.
Pune
2009.10 - 2017.06
My work responsibilities included
- Gene cloning and protein expression bacterial and baculovirus expression systems
- Stable cell line development for various drug discovery projects
- Bio-assay optimization & validation.
Senior Scientist
baculovirusmAbsSyngene International Ltd
Bangalore
2007.08 - 2009.10
My work profile includes
- Monoclonal antibody development ( Includes animal immunization, hybridoma development,Monoclonal antibody expression)
- VL and VH cloning and expression in cell lines. (Includes mRNA isolation, CDNA synthesis, gene cloning, sequence analysis, cloning into an expression vector.)
- Stable cell line development for mAB production.
Research Associate
Shantha Biotechnics (SANOFI, India)
Hyderabad
2005.01 - 2007.07
My work responsibilities included
- Monoclonal antibody development
- Stable cell line development
Education
Ph.D. - Biotechnology
JNTU
HyderabadSkills
- Molecular biology
- Cell line development
- Monoclonal antibody discovery
- Antibody engineering
- Cell line engineering
- Emboli and Insect cell expression
- Purification
Academic Profile
JNTU, Hyderabad, Ph.D. in Biotechnology, Cloning, expression and purification of catalytic subunit of human and bovine enteropeptidase-its influence on NF-κB signaling, Barkatullah University, Bhopal,
M.Sc. in Biotechnology
Profile Snapshot
Researcher with 19+ years of industrial experience in small molecule and large molecule drug discovery.
- Hands on experience in Stable cell line development for antibody production in CHO cell lines by DHFR amplification and GS selection methods
- Experienced in Cloning, Protein expression and Purification in bacterial, baculovirus and mammalian systems.
- Expertise in Monoclonal antibody generation by hybridoma and phage display
- Actively involved in new drug discovery research specifically in the area of pain and cancer.
Strong capabilities in training, team development and lab management
Personal Information
- Father's Name: Abdul Rahman
- Nationality: Indian
- Marital Status: Married
- Religion: Islam
Languages
- English
- Urdu
- Telugu
- Hindi
Publications
TransasiaBiotechnicsShanthaLupinRahman, 2014
A Quick and Efficient Method to Quantify Baculovirus by Quantitative Real-Time PCR, BIOSCIENCES BIOTECHNOLOGY RESEARCH ASIA, 10, 2, 659-663, 2013, 10.13005/bbra/1178
enterokinaseMicrobiolEnvEnterokinaseBaculovirusbbra
Timeline
Asst. General Manager
Transasia Bio-Medical Pvt Ltd
2021.08 - Current
Principal Investigator
Syngene International Ltd
2017.07 - 2021.07
Research Scientist
Lupin Ltd.
2009.10 - 2017.06
Senior Scientist
baculovirusmAbsSyngene International Ltd
2007.08 - 2009.10
Research Associate
Shantha Biotechnics (SANOFI, India)
2005.01 - 2007.07
Ph.D. - Biotechnology
JNTU
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