Mohammed Azhar

Head-Monoclonal antibody and Protein generation

My work responsibilities includes

• Monoclonal antibody generation through Hybridoma and Phage display technology
• Protein expression through Emboli, Pichia, Insect and mammalian expression systems
• Stable cell line development by Glutamine synthetase expression system in CHO-K1 cells
• Purification of antibodies and proteins
• Team management.

As a group leader of the protein expression group, my work responsibilities includes

• Stable cell line development for expressing different formats of mAbs in Piggyback expression system (from mg to grams level).
• Mammalian display platform establishment by Lent virus method.

My work responsibilities included

• Gene cloning and protein expression bacterial and baculovirus expression systems
• Stable cell line development for various drug discovery projects
• Bio-assay optimization & validation.

My work profile includes

• Monoclonal antibody development ( Includes animal immunization, hybridoma development,Monoclonal antibody expression)
• VL and VH cloning and expression in cell lines. (Includes mRNA isolation, CDNA synthesis, gene cloning, sequence analysis, cloning into an expression vector.)
• Stable cell line development for mAB production.

My work responsibilities included

• Monoclonal antibody development
• Stable cell line development