Subhash Bathini

Results-driven and client-focused technical sales representative with a strong emphasis on building and nurturing business relationships. Demonstrated ability to find effective solutions to customer issues, leading to long-term client partnerships. Prepared to utilize technical expertise and sales acumen to drive business growth in a technology sales-oriented role within an organization. With extensive experience delivering top-notch solutions, I recognize the significance of providing exceptional service to foster long-term client loyalty.

• TECHNO COMMERCIAL ROLE, Responsible for Sales and Technical Support for Cell Therapy (CAR T), Gene Therapy, Biologics Development and Manufacturing to Indian Biotech/Life science customers. Deliver product-and workflow- specific consultations relating to the Cell Therapy Systems (CTS) portfolio of closed, modular cell processing systems and GMP grade ancillary reagents. Provide independent on-site and remote scientific application training and support. Support pre-sales demos and post-sales product training. Develop and lead hands-on workshops, demos, and training. Provide market sensing mechanism, identifying new opportunities and trends, and highlighting gaps in current portfolio. Work with internal teams to develop and update workflow applications and education content for customer training in the form of videos, seminars, training manuals etc. Connect with the global Field Applications and Technical Sales teams and share processes across the organization. Work with the global and local R&D members to lead, facilitate and follow through with the implementation of new projects with clients. Actively plan and participate in conducting capability presentations, technical discussions and seminars for customers. Troubleshoots existing issues and suggests solutions to both R&D and Commercial clients. Experience in managing multiple projects, developing and communicating ideas and designing marketing plans to achieve goals. Proficient at listening and understanding customer requirements, providing solutions and building effective business relationships. My success is due to being professional, organized and adaptable in a fast paced, competitive and constantly changing environment. Strategic Consulting, including business plan & sales strategy development Documentation and Regulatory support.
• CELL THERAPY AND GENE THERAPY, Closed, End to End CAR T Manufacturing workflow demonstrations at onsite. Hands on experience on CTS Rotea, CTS Dynacellect system, CTS DynaMag, CTS Dynabeads and CTS Detachable Dynabeads, G-rex 100M vessels and Optimizer Pro Media system. Demonstrate PBMC Isolation from Buffy coat using CTS Rotea System. Isolate and Activate T cells using CTS Dynacellect system. Expansion of T Cells in appropriate medium with IL2 and ICSR supplementation. Remove beads using CTS Dynacellect de-beading protocol and formulate T cells in freezing media. Results and Data Interpretation during process – Isolation efficiency, Cell counts, Viability, Cell Phenotype, CD 19 CAR expression, Activation Markers and Exhaustion Markers profile analysis. Viral vectors production solutions – AAV MAX and LV MAX systems. Plasmid production and HEK media panel solutions.
• MAMMALIAN CELL CULTURE, Hands on experience on multiple cell lines CHO S, CHO DG44, BHK-21, CHO E, CHO K1, HEK 293 6E and HEK 293 F, Expi 293 and Expi CHO and Insect cell lines (HI-5, SF9 and Super SF9) and their maintenance. Stable cell line generation and Transient Expression. Stable cell line generation. (Pool generation, Limiting dilution, Screening of clones and generation of final single clones). Transient Transfection and Stable Transfection. Stable Transfection using: Electroporation, Freestyle Max, Lipofectamine. Transient Transfection using: PEI, Lipofectamine, Fecto pro and TransIT pro. Done stable cell line clone development in both suspension and adherent cell lines in different cell lines. Small scale expression checks to see the expression levels. Study on different parameters to increase the expression levels like DNA, Transfection Reagent, Temperature shift and different Feeds. Hands on experience on Wave Bioreactors and Glass bioreactors (sartorious, GE and PALL) and able to take Scale up batches like 5L, 10L,20L and 50L independently using Single use disposable bags. Worked on different insect cell lines (Hi5, SF9 and Super SF9). Screening of clones by ELISA, Octet and FACS and MTT ASSAY. Clarification and filtration of Harvest. Purification of proteins in small scale up to 1L. Optimization studies at shake flask level, fermentation in 5L dual bioreactor and scale up to 40L dual bioreactor, kLa estimation. SIP (Sterilization in Place) – ESIP and FSIP, CIP (Cleaning in Place), Integrity testing for filters, heat penetration studies for Dry heat sterilizer and autoclave.
• MOLECULAR BIOLOGY, Cloning, Restriction digestion, Ligation, Competent cell preparation, Transformation and Screening techniques and Restriction Mapping. Scale up of DNA: MiniPrep, Maxi Prep, Mega Prep and Giga Prep. Purity and Concentration determination of DNA by NanoDrop. Polymerase Chain Reaction (PCR)

• English
• Hindi
• Telugu
• Kannada

• Date of Birth: 09/24/86
• Gender: Male
• Marital Status: Married